Naslov
Nicotine content in transformed haploid and dihaploid tissues of tobacco (Nicotiana tabacum L.)

Autori
Ćurković Perica, Mirna ; Gillet, Francoise ; Jacquin-Dubreuil, Annie ; Krsnik-Rasol, Marijana ; Jelaska, Sibila

Izvornik
Phyton : annales rei botanicae (0079-2047) 37 (1998), 2; 229-239

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
dihaploids; haploids; Nicotiana tabacum; nicotine content; transformation

Sažetak
Three anther-derived haploid and three haploid-derived dihaploid tissue lines of tobacco were transformed with three strains of Agrobacterium tumefaciens: B6S3, pGV2215 and pGV2255. Unorganized, shooty and rooty tumours were induced, respectively. Tumour morphology and the growth rate of transformed tissues depended mainly on the bacterial strain. The presence of T-DNA genes in transformed tissues was confirmed by polymerase chain reaction (PCR). By means of high performance liquid chromatography (HPLC) nicotine was found only in the control plants and in the roots transformed with Agrobacterium tumefaciens pGV2255. Its content did not depend on the ploidy level of the tissue. Transformed roots could not be subcultured because of callogenesis. The callus which overgrew transformed roots did not produce nicotine.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA