Naslov
Photoaffinity glycoprobes - a new tool for the identification of lectins

Autori
Lauc, Gordan ; Lee, Reiko ; Lee, Yuan

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
18th International Congress of Biochemistry and Molecular Biology Abstract Book / - Birmingham : IUBMB, 2000

Skup
18th International Congress of Biochemistry and Molecular Biology Young Scientist Travel Fellowship Symposium

Mjesto i datum
Birmingham, Ujedinjeno Kraljevstvo, 13.07.2000. - 16.07.2000

Vrsta sudjelovanja
Pozvano predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
glycoprobes; lectins; juvenile rheumatoid arthritis

Sažetak
One of the proposed functions for the carbohydrate structures on glycoconjugates is the transfer of information through interaction with specific lectin receptors. However, the number of elucidated functional lectin-carbohydrate interactions is still relatively small, largely due to the lack of adequate methods to identify lectin activity in complex biological samples. Aiming to solve this problem, we have developed a method based on the novel group of compounds we named glycoprobes. The glycoprobe consists of three vital parts: (i) glycan ; (ii) digoxin tag ; and (iii) photoreactive crosslinker. When incubated in dark, oligosaccharide part of the glycoprobe forms a complex with lectin. After illumination, covalent link between the probe and the lectin is formed resulting in a digoxin-tagged lectin. Using antibodies against digoxin, this complex can easily be identified immuno/cytochemically, or by Western blots. To demonstrate the applicability of glycoprobes we have used Man$_{9}-glycoprobe (containing Man$_{9} oligosaccharide) and YEE(ahGalNAc)$_{3}-glycoprobe (containing a synthetic neoglycopeptide with three terminal N-acetylgalactosamine residues) to identify lectins in bovine serum and rat liver membranes. The simplicity of the method enables its application in routine monitoring of changes in lectin activity during various developmental or pathological processes. An example of GalNAc-binding analysis in human serum is shown.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Javno zdravstvo i zdravstvena zaštita

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