Naslov
Biotechnology of human skin cell cultivation - logistic aspects and infrastructural support

Autori
Boranić, Milivoj ; Fattorini, Ivan ; Vrtar, Zvonimir

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, cjeloviti rad (in extenso), pregledni

Izvornik
Current studies in Biotechnology, Volume I - Biomedicine / Kniewald, Zlatko i sur. - Zagreb : Croatian Society for Biotechnology, 2000, 29-32

Skup
Scientific Conference Biotechnology and Biomedicine

Mjesto i datum
Zagreb, Hrvatska, 22.02.1999. - 23.02.1999

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Domaća recenzija

Ključne riječi
human keratinocytes; cells cultured; biotechnology; burns

Sažetak
Extensive skin defects after burns are covered by autologous or allogeneic skin transplants, lyophilized animal skin, artificial tissues made of biodegradable material or skin epithelial cells (keratinocytes) cultured in vitro. Autologous keratinocytes obtained from a skin biopsy may in three weeks yield progeny sufficient to cover a large body area of a burned patient. The period required for the expansion of the keratinocyte cultures is bridged by temporary transplants of allogeneic skin or artificial material. Cultivation of human keratinocytes has been started as a pilot project in our Institute and the first cultures have been obtained. So far the cultures were used for the research purposes. Clinical application of cultured keratinocytes is planned to take place in collaboration with plastic surgeons. Logistic prerequisites for the production and clinical use of cultured keratinocytes include: appropriate laboratory space and equipment, specifically educated laboratory personnel, safety testing of the cultured material, ethical committee permission for clinical testing of the cultured material, a trained surgical and supportive care team, and financial background.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti

POVEZANOST RADA