Naslov
Validation of QTLs for Orobanche crenata resistance in faba bean (Vicia faba L.) across environments and generations

Autori
Diaz-Ruiz, Ramon ; Torres, Ana Maria ; Šatović, Zlatko ; Gutierrez, Maria Victoria ; Cubero, Jose Ignacio ; Roman, Belen

Izvornik
Theoretical and applied genetics (0040-5752) 120 (2010), 5; 909-919

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
broomrape; resistance breeding; marker assisted selection; F2; RILs; QTL evaluation

Sažetak
Broomrape (Orobanche crenata Forsk.) is a major root–parasite of faba bean (Vicia faba L.), that seriously limits crop cultivation in the whole Mediterranean area. This parasitic weed is difficult to control, difficult to evaluate and the resistance identified so far is of polygenic nature. This study was conducted to identify genetic regions associated with broomrape resistance in recombinant inbred lines (RILs) and to validate their previous location in the original F2 population derived from the cross between lines Vf6 and Vf136. A progeny consisting of 165 F6 RILs was evaluated in three environments across two locations in 2003 and 2004. Two hundred seventy seven molecular markers were assigned to 21 linkage groups (9 of them assigned to specific chromosomes) that covered 2, 856.7 cM of the V. faba genome. The composite interval mapping on the F6 map detected more quantitative trait loci (QTL) than in the F2 analysis. In this sense, four QTLs controlling O. crenata resistance (Oc2–Oc5) were identified in the RI segregant population in three different environments. Only Oc1, previously reported in the F2 population, was not signiWcant in the advanced lines. Oc2 and Oc3 were found to be associated with O. crenata resistance in at least two of the three environments, while the remaining two, Oc4 and Oc5, were only detected in Córdoba-04 and Mengíbar-04 and seemed to be environment dependent.

Izvorni jezik
Engleski

Znanstvena područja
Poljoprivreda (agronomija), Biotehnologija

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