Naslov
Insulin administration in the mild hyperglycaemia changes expression of proinflammatory adhesion molecules on human aortic endothelial cells

Autori
Bačun, Tatjana ; Belovari, Tatjana ; Suver, Mirjana ; Dobrošević, Blaženka ; Mihaljević, Ivan ; Glavaš-Obrovac, Ljubica

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
HDBMB 2008 Congress of the Croatian Society of Biochemistry and Molecular Biology with international participation : Book of abstracts / Strelec, Ivica ; Glavaš-Obrovac, Ljubica - Zagreb : Hrvatsko Društvo za Biotehnologiju, 2008, 63-63

ISBN
978-953-95551-2-0

Skup
Congress of the Croatian Society of Biochemistry and Molecular Biology with international participation

Mjesto i datum
Osijek, Hrvatska, 17.09.2008. - 20.09.2008

Vrsta sudjelovanja
Poster

Vrsta recenzije
Nije recenziran

Ključne riječi
Cell adhesion molecules; mild hyperglycaemia; insulin; human aortic endothelial cells

Sažetak
The induction of adhesion molecules on the surface of endothelial cells is one of the first steps in a high glucose-mediated endothelial dysfunction in diabetic patients. Influence of insulin administration in condition of elevated glucose concentration on the E-selectin, vascular adhesion molecule-1 (VCAM-1), and intracellular adhesion molecule-1 (ICAM-1) expression on human aortic endothelial cells (HAEC) was investigated. HAEC were cultured 4 h in a Medium 200 with low serum grow supplement, homocysteine (7 μM) and glucose in range of concentration (5.5, 8.0, 12.0 and 16.5 mM). Expression of CAMs were analysed by flow-cytometry using monoclonal antibodies. Controls were CAMs expression in the medium with a physiological glucose concentration (5.5 mM). The influence of insulin (1 mUI/mL) was analyzed in combination with each glucose concentration. Incubation of HAEC with 8 mM glucose increased expression of E-selectin for 2-fold, ICAM-1 for 47% and VCAM-1 for 3-fold. Expression of E-selectin was 23% lesser, ICAM-1 was 64% higher and VCAM-1 was 41% when cells were grown with 12 mM glucose. Incubation of HAEC with 16.5 mM glucose decreased expression of E-selectin for 88%, ICAM-1 for 12% and VCAM-1 for 43%. Addition of insulin was not changed expression of CAMs expression in a medium with 5.5 mM glucose. In conditions of elevated glucose concentration (12 mM), addition of insulin resulted in significantly decreased expression of E-selectin (27%) and increased VCAM-1 (23%) expression. In conclusion, moderate higher glucose concentration increased E-selectin, ICAM-1 and VCAM-1 expression in HAEC. Insulin administration in the mild hyperglycaemia condition reduces the expression of the proinflammatory adhesion molecule E-selectin which could contribute in slowing of macrovascular complications development in diabetic patients.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Biologija, Temeljne medicinske znanosti

POVEZANOST RADA