Naslov
A novel detection approach based on coupled redox-complexation reaction with 2, 4, 6-Trypyridyl-s-triazine (TPTZ) as an chromogenic reagent and its application to flow-injection spectrophotometric determination of tiopronin {;N-(2-Mercaptopropionyl)-Glycine};

Autori
Kukoč Modun, Lea ; Radić, Njegomir

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Euroanalysis 2009, Abstracts / Buchberger, Wolfgang ; Lindner, Wolfgang - Innsbruck : Asac, 2009, 749-749

Skup
Euroanalysis 2009

Mjesto i datum
Innsbruck, Austrija, 04.09.2009. - 09.09.2009

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
tiopronin; N-(2-Mercaptopropionyl)-Glycine; Flow-injection spectrophotometry; pharmaceuticals

Sažetak
A flow injection spectrophotometric procedure for N-(2–mercaptopropionyl)-glycine (MPG) determination is proposed. The determination is based on coupled redox-complexation reaction between MPG, Fe(III) and 2, 4, 6-Trypyridyl-s-triazine (TPTZ). Firstly MPG reduces Fe(III) to Fe(II) and the reduced Fe(II) is rapidly converted to the highly stable, deep-blue colored Fe(TPTZ)22+. This colored complex was monitored at 593 nm. Optimal conditions for determining MPG are achieved by univariate method. A linear calibration curve is established in a concentration range of 4.0  10-6 to 2.0  10-4 mol L-1 MPG with regression equation y = 4704 x - 0.019 (R2 = 0.9998) (n = 10) and detection limit of 2.35  10-7. The analytical frequency was 60 h-1. The proposed method is simple, rapid, sensitive and reproducible (RSD 0.5%, n = 10), and can be applied for determination of MPG in pharmaceutical preparations up to nanomole quantity. Usual excipients used as additives in pharmaceuticals do not interfere with the proposed method.

Izvorni jezik
Engleski

Znanstvena područja
Kemija

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