Pregled bibliografske jedinice broj: 442672
Comparison of Cytotoxicity Induced by 17α-Ethynylestradiol and Diethylstilbestrol in Fish CCO and Mammalian CHO-K1 Cell Lines
Comparison of Cytotoxicity Induced by 17α-Ethynylestradiol and Diethylstilbestrol in Fish CCO and Mammalian CHO-K1 Cell Lines // Bulletin of environmental contamination and toxicology, 86 (2011), 3; 252-257 doi:10.1007/s00128-011-0217-z (međunarodna recenzija, članak, znanstveni)
CROSBI ID: 442672 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Comparison of Cytotoxicity Induced by 17α-Ethynylestradiol and Diethylstilbestrol in Fish CCO and Mammalian CHO-K1 Cell Lines
Autori
Radošević, Kristina ; Tonković, Tea ; Slivac, Igor ; Kniewald, Zlatko ; Gaurina Srček, Višnja
Izvornik
Bulletin of environmental contamination and toxicology (0007-4861) 86
(2011), 3;
252-257
Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni
Ključne riječi
CCO cells; CHO-K1 cells; Cytotoxicity; Synthetic estrogens
Sažetak
The effects of synthetic estrogens 17α-ethynylestradiol (EE2) and diethylstilbestrol (DES) were compared on cell proliferation and morphology in Channel Catfish Ovary (CCO) and Chinese Hamster Ovary (CHO-K1) cells. EE2 exposure (0.1 and 0.5 µg/mL) induced stimulatory effect on CCO and CHO-K1 cell proliferation, while higher concentrations (1-10 µg/mL) showed cytotoxic effects. Increase in DES concentrations mainly resulted in dose-depended increase in cytotoxicity in both cell lines. Morphological changes induced by EE2 and DES exposure (5µg/mL) showed disrupted cell monolayer and increased number and size of lysosomes. Comparison of IC50 values showed almost equal sensitivity towards cytotoxicity of tested compounds in CCO and CHO-K1 cells.
Izvorni jezik
Engleski
Znanstvena područja
Biotehnologija
POVEZANOST RADA
Projekti:
058-0582184-2414 - Nekliničke metode ispitivanja tvari iz okoliša na stanicama sisavaca i riba (Gaurina-Srček, Višnja, MZOS ) ( CroRIS)
Ustanove:
Prehrambeno-biotehnološki fakultet, Zagreb
Profili:
Kristina Radošević
(autor)
Višnja Gaurina-Srček
(autor)
Zlatko Kniewald
(autor)
Igor Slivac
(autor)
Citiraj ovu publikaciju:
Časopis indeksira:
- Current Contents Connect (CCC)
- Web of Science Core Collection (WoSCC)
- Science Citation Index Expanded (SCI-EXP)
- SCI-EXP, SSCI i/ili A&HCI
- Scopus
- MEDLINE
