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Pregled bibliografske jedinice broj: 442611

Decidualne CD14+ antigen predočne stanice jesu alternativno aktivirane


Sršen Medančić, Suzana; Laškarin, Gordana; Redžović, Arnela; Vlastelić, Ivan; Rukavina, Daniel
Decidualne CD14+ antigen predočne stanice jesu alternativno aktivirane // Zbornik Radova/Abstracts Book / Stipić Marković, Asja ; Čvorišćec, Branimir (ur.).
Zagreb: Hrvatsko društvo za alergologiju i kliničku imunologiju, 2009. str. 143-144 (poster, domaća recenzija, sažetak, znanstveni)


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Naslov
Decidualne CD14+ antigen predočne stanice jesu alternativno aktivirane
(Decidual CD14+ antigen presebting cells are alternatively activated)

Autori
Sršen Medančić, Suzana ; Laškarin, Gordana ; Redžović, Arnela ; Vlastelić, Ivan ; Rukavina, Daniel

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Zbornik Radova/Abstracts Book / Stipić Marković, Asja ; Čvorišćec, Branimir - Zagreb : Hrvatsko društvo za alergologiju i kliničku imunologiju, 2009, 143-144

ISBN
978-953-6201-15-0

Skup
Prvi kongres hrvatskih alergologa i kliničkih imunologa s međunarodnim sudjelovanjem

Mjesto i datum
Zagreb, Hrvatska, 21.05.2009. - 23.05.2009

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
decidualne CD14+ stanice; rana trudnoća; periferna krv
(decidual CD14+ cells; early pregnancy; peripheral blood)

Sažetak
The aim was to explore the activation status of early decidual CD14+ cells in terms of surface markers expression, cyto/chemokine production, endocytic capability and lipopolysaccharide (LPS) response. Methods of the study: Labeling of CD14 and CD209 antigens was performed by immunohistology in the first trimester normal human pregnancy deciduas. Early pregnancy decidual mononuclear cells (DMC) were obtained by collagenase digestion and subsequent gradient density centrifugation. Gradient density separation was sufficient to obtain peripheral blood leukocytes. Flow cytometry was used to analyze phenotype and intracellular cyto/chemokine expression in un-stimulated or LPS stimulated CD14+ cells from deciduas or peripheral blood samples of the same women. Chemokine secretion was measured by ELISA in the supernatant of decidual magnetically enriched CD14+ cells. Endocytic activity was determinated by FITC-Dextran uptake using Flow cytometry in CD14+ cells, un-treated or treated with increasing concentrations of LPS. Results: Decidual and peripheral blood CD14+ cells expressed negligible percentage of CD1a and CD83 markers what distinguishes them from early dendritic cell differentiation stages. Decidual CD14+ cell population showed higher percentage of chemokine CC receptor 5 (CCR5) and decoy receptors (CD206, CD209, CD163), but they do not express CD16 in comparison to peripheral blood CD14+ cells. The dominance of IL-4+ over IFN-+ cells and CCL22+ over CCL3+ cells in decidual CD14+ cells was found. The secretion of CCL17 and CXCL10 was balanced. Decidual CD14+ cells internalized FITC-dextran efficiently, but they were incapable to decrease endocytosis or increase IFN- production after 4 hrs LPS - stimulation. Conclusions: Decidual CD14+ cells represent autentic decidual population, which is probably settled in decidual tissue from the peripheral blood due to their pro-inflammatory CCR5 expression. This population seemed to be alternatively activated within decidual tissue due to decoy receptor and CD16 expression pattern, as well as unresponsiveness to LPS stimulation. Intracellular cyto/chemokine production and secretion by decidual CD14+ cells suggest their anti-inflammatory orientation. Acknowledgement: The experiments were financed by Croatian Ministry of Science, Education and Sports Grants No. 0620402-0376 and No. 062-620402-0377 and the investigation was performed at Medical Faculty, University of Rijeka

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti



POVEZANOST RADA


Projekti:
062-0620402-0376 - Citokini i citolitički mehanizmi tijekom rane trudnoće (Rukavina, Daniel, MZOS ) ( CroRIS)
062-0620402-0377 - Imunoregulacijske funkcije antigen predočnih stanica tijekom rane trudnoće (Laškarin, Gordana, MZOS ) ( CroRIS)
062-0620402-0379 - Imunološki mehanizmi u žena s patološkim trudnoćama (Haller, Herman, MZOS ) ( CroRIS)

Ustanove:
Medicinski fakultet, Rijeka


Citiraj ovu publikaciju:

Sršen Medančić, Suzana; Laškarin, Gordana; Redžović, Arnela; Vlastelić, Ivan; Rukavina, Daniel
Decidualne CD14+ antigen predočne stanice jesu alternativno aktivirane // Zbornik Radova/Abstracts Book / Stipić Marković, Asja ; Čvorišćec, Branimir (ur.).
Zagreb: Hrvatsko društvo za alergologiju i kliničku imunologiju, 2009. str. 143-144 (poster, domaća recenzija, sažetak, znanstveni)
Sršen Medančić, S., Laškarin, G., Redžović, A., Vlastelić, I. & Rukavina, D. (2009) Decidualne CD14+ antigen predočne stanice jesu alternativno aktivirane. U: Stipić Marković, A. & Čvorišćec, B. (ur.)Zbornik Radova/Abstracts Book.
@article{article, author = {Sr\v{s}en Medan\v{c}i\'{c}, Suzana and La\v{s}karin, Gordana and Red\v{z}ovi\'{c}, Arnela and Vlasteli\'{c}, Ivan and Rukavina, Daniel}, year = {2009}, pages = {143-144}, keywords = {decidualne CD14+ stanice, rana trudno\'{c}a, periferna krv}, isbn = {978-953-6201-15-0}, title = {Decidualne CD14+ antigen predo\v{c}ne stanice jesu alternativno aktivirane}, keyword = {decidualne CD14+ stanice, rana trudno\'{c}a, periferna krv}, publisher = {Hrvatsko dru\v{s}tvo za alergologiju i klini\v{c}ku imunologiju}, publisherplace = {Zagreb, Hrvatska} }
@article{article, author = {Sr\v{s}en Medan\v{c}i\'{c}, Suzana and La\v{s}karin, Gordana and Red\v{z}ovi\'{c}, Arnela and Vlasteli\'{c}, Ivan and Rukavina, Daniel}, year = {2009}, pages = {143-144}, keywords = {decidual CD14+ cells, early pregnancy, peripheral blood}, isbn = {978-953-6201-15-0}, title = {Decidual CD14+ antigen presebting cells are alternatively activated}, keyword = {decidual CD14+ cells, early pregnancy, peripheral blood}, publisher = {Hrvatsko dru\v{s}tvo za alergologiju i klini\v{c}ku imunologiju}, publisherplace = {Zagreb, Hrvatska} }




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