Naslov
The interaction between decidual CD14+ antigen presenting and NK cells under the influence of mucin 1.

Autori
Laškarin, Gordana ; Sršen-Medančić, Suzana ; Redžović, Arnela ; Vlastelić, Ivan ; Rukavina, Daniel

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Abstracts Book / Stipić Marković, Asja ; Čvorišćec, Branimir - Zagreb : Hrvatsko društvo za alergologiju i kliničku imunologiju, 2009, 127-128

ISBN
978-953-6201-15-0

Skup
First Croatian Congress of Allergology and Clinical Immunology with International Participation

Mjesto i datum
Zagreb, Hrvatska, 21.05.2009. - 23.05.2009

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
mucin 1; decidualne CD14+ stanice; rana trudnoća
(mucin 1; decidual CD14+ cells; early pregnancy)

Sažetak
Although mucin 1 (MUC 1) removal from uterine epithelial cells is precondition for successful implantation, its immunological role within decidua is largely unknown. We have previously shown that decidual CD14+ cells bind and internalize MUC 1 by CD206 and CD209 receptors on a dose dependent manner. In this work we investigated the influence of MUC 1 on the function of decidual CD14+ cells, particularly in terms of CD14+/CD56+ cells interaction. CD14+ cells recovered from the suspension of early pregnancy decidual mononuclear cells (DMC) treated with MUC 1 (200 µg/ml) did not basically change CD80, CD86, HLA-DR and CD91 markers and decoy receptors (IL-1 receptor type II and D6) expressions, as it was measured by flow cytometry. Stimulation of DMC with MUC 1 did not affect the expression of IFN-, IL-4 and IL-10 cytokines inCD14+ cells, as it was detected by intracellular cytokine labeling using immunofluorescence and flow cytometry. Intracellular production of IL-15, responsible for proliferation and cytotoxic mediator expression in NK cells, decreased in MUC 1 treated CD14+ cells. MUC 1 treated CD14+ cells were unable to sustain proliferation of magnetically purified decidual CD56+ cells measured by CFSE proliferation assay, and perforin and FasL cytotoxic molecules expression after 18 hrs co-culture at CD14+/CD56+ cell ratio 1:5, as untreated CD14+ cells did. The expression of chemokine CCL17 increased in CD56+ cells after the co-culture with MUC1 treated CD14+ cells in comparison to untreated CD14+ cells, whereas chemokines CCL3 and CCL22, and cytokines IL-4 and IFN- remained unchanged. If the MUC 1 is present in the uterine mucosa, it might affect decidual CD14+ cells function and hamper physiological pro-inflammatory response during implantation. It could lead to inadequate trophoblast growth control due to decreased number of decidual CD56+ NK cells less endowed with cytotoxic mediators. Acknowledgements: The experiments were financed by the grants of Croatian Ministry of Science No. 062-620402-0376 and No. 062-620402-0377.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti, Kliničke medicinske znanosti

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