Naslov
The effects of splenectomy and autologous spleen transplantation on total white blood cell count, differential leukocyte count and cell morphology in pigs

Autori
Poljičak-Milas, Nina ; Migić, Josipa ; Vnuk, Dražen ; Milinković-Tur, Suzana ; Marenjak, Terezija Silvija

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Inflammation 2010, Inflammatory cell signaling mechanisms as therapeutic targets / Marc Diederich - Luxembourg : Recherches Scientifiques Luxembourg, 2010, 253-253

Skup
Inflammation 2010, Inflammatory cell signaling mechanisms as therapeutic targets

Mjesto i datum
Luksemburg, 27.01.2010. - 30.01.2010

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Splenectomy; Autotransplantation; White blood cell count; Cell morphology; Pigs

Sažetak
Subject of this research were nineteen Landras piglets aged 3 months. After induction of anesthesia piglets were randomly divided in to three groups: sham-operation (n=6), total splenectomy (n=6), and splenic autotransplantation (n=7) with spleen chips autotransplanted into the greater omentum. The blood samples were taken just before the surgery, and on 1st, 5th, 12th, 26th and 40th postoperative day. EDTA blood samples were used to determine total white blood cell count and blood smears were made to perform differential leukocyte count and morphologic evaluation of the cells. All groups showed leukocytosis following the operation but this was not regarded as a change specific for splenectomy or autotransplantation, rather than a post-injury inflammatory response due to tissue lesions during operation. In addition, up to 12th postoperative day, increase of the absolute differential number of band neutrophils and sporadic findings of granulocyte precursors and dividing cells were found, suggesting an increased bone marrow activity and release of immature neutrophils and their mobilization from the marginal pool. Increase of the absolute neutrophil number and initial decrease of the lymphocyte number was found in all groups, followed by the later recovery of the lymphocyte number. But, no unique pattern was determined because these changes had different onset time and intensity in each group. Described variation in number of neutrophils and lymphocytes was typical for the physiological immune response of the peripheral white blood cells to different stress factors, and correlate with the patients’ clinical status. Inflammatory/immune response caused by stress factors can be effectively evaluated using a Neutrophil-lymphocyte stress factor (NLSF), and in this research, the highest values were found in the group of splenectomized piglets. Postoperative recovery of lymphocyte number was slowest in this group, which could indicate a possible lymphopoethic function of the autotransplanted splenic chips. Morphological evaluation revealed some reactive, granulated and cytotoxic lymphocytes on peripheral blood smears of all groups on the 1st and 5th day after the surgery. On the 12th day granulated lymphocytes were frequently found in the group with autotransplantated splenic chips. These morphological changes were probably caused by the alarmins release from the destructed and autotransplanted cells, which triggered the reaction of humoral and cellular immunity.

Izvorni jezik
Engleski

Znanstvena područja
Veterinarska medicina

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