Naslov
Time-course expression of metallothioneins and tissue metals in chronic relapsing experimental autoimmune encephalomyelitis in DA rats

Autori
Jakovac, Hrvoje ; Grebić, Damir ; Tota, Marin ; Barac-Latas, Vesna ; Mrakovčić-Šutić, Ines ; Milin, Čedomila ; Radošević-Stašić, Biserka

Izvornik
Histology and histopathology (0213-3911) 26 (2011), 2; 233-245

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
chronic relapsing experimental autoimmune encephalomyelitis; metallothioneins I+II; zinc; copper; CNS; liver.

Sažetak
To elucidate the role of metallothioneins (MTs) in the pathomechanisms of autoimmune CNS disorders we estimated the expression of MTs I+II and the tissue concentrations of Zn2+ and Cu2+ in the brain, spinal cord (SC) and in the liver during the periods of attacks and remissions in chronic relapsing experimental autoimmune encephalomyelitis (CR-EAE). Disease was induced in the genetically susceptible Dark Agouti (DA) rats by subcutaneous injection of bovine brain homogenate in CFA. Control rats were treated with CFA or with saline solution. The data, obtained by clinical assessment, immunohistochemistry and inductivity coupled plasma spectrometry, have shown that during the first attack (12th day) MTs I+II were markedly upregulated in subarachnoid regions and perivascular space on astrocytes, microglia and spinal neurons. Simultaneously, increased the concentration of zinc in the SC and zinc and copper in the liver. During the second attack (22nd day) a new overexpression of MTs was found in the cerebellum, in sulcus hippocampi, in spinal neurons and particularly on hepatocytes around the central vein. Concomitantly, in the brain and SC increased the concentration of copper. The data point to neuroprotective role of MTs and to important regulatory role of essential metals and hepatic MTs in the pathogenesis of CR-EAE

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

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