Naslov
Antifibrotic activity of Taraxacum officinale root in carbon tetrachloride-induced liver damage in mice

Autori
Domitrović, Robert ; Jakovac, Hrvoje ; Romić, Željko ; Rahelić, Dario ; Tadić, Žarko

Izvornik
Journal of ethnopharmacology (0378-8741) 130 (2010), 3; 569-577

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
carbon tetrachloride; liver fibrosis; dandelion (taraxacum officinale); metallothionein; glial fibrillary acidic protein; α-smooth muscle actin

Sažetak
Dandelion (Taraxacum officinale) has been traditionally used in the treatment of various liver disorders. The present study was aimed to assess the efficacy of dandelion root water-ethanol extract (DWE) in carbon tetrachloride (CCl4)-induced hepatic fibrosis. The mice were treated with CCl4 dissolved in olive oil (20% v/v, 2 mg/kg) intraperitoneally (i.p.), twice a week for 4 weeks. DWE was administered i.p. once daily for next 10 days, in doses of 200 and 600 mg/kg of body weight. Hepatic Cu/Zn SOD activity was decreased in intoxicated mice and normalized in DWE treated groups. Metallothionein (MT) I/II immunopositivity was strongly reduced in the CCl4 group. DWE treatment successfully decreased hepatic fibrinous deposits, restored histological architecture, and modulate the expression glial fibrillary acidic protein and α-smooth muscle actin. Concomitantly, MT I/II expression increased in the DWE treated groups. Our results suggest the therapeutic effect of DWE on CCl4-induced liver fibrosis by the inactivation of hepatic stellate cells and the enhancement of hepatic regenerative capabilities. The present results provide scientific evidence to substantiate the traditional use of Taraxacum officinale in hepatic disorders.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti, Farmacija

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