Naslov
Molecular identification and antimicrobial susceptibility of Champylobacter strains isilated from chickens in Croatia

Autori
Prukner-Radovčić, Estella ; Horvatek, Danijela

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
XVIth World Veterinary Poultry Association Congress ; Book of abstracts / - Marakeš : World Veterinary Poultry Association, 2009

Skup
XVIth World Veterinary Poultry Association Congress

Mjesto i datum
Marrakesh, Maroko, 08.11.2009. - 12.11.2009

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Molecular identification; antimicrobial susceptibility; Champylobacter; chickens; Croatia

Sažetak
The aim of the study was to evaluate the presence of clinically relevant Campylobacter spp. in poultry in Croatia. The zoonosis campylobacteriosis, caused by thermophilic Campylobacter spp., is an important public health problem in most areas of the world. Poultry meat and products appears to be a major source of campylobacteriosis through cross-contamination of ready-to-eat foods, direct hand-to-mouth transfer during food preparation, and to a lesser extent, through the consumption of undercooked poultry meat. Campylobacteriosis is the second reported food-borne infection in Croatia but the national action plan for surveillance of campylobacter doesn’t exist, and epidemiological studies weren’t conducted so far to reveal the real prevalence of these bacteria in poultry. Cloacal swabs were taken on the farms from altogether 91 broiler chickens and 28 laying hens at the end of the laying period. All swabs were subjected first to bacteriological examination using modified Charcoal Cefoperazone Desoxycholate Agar (mCCDA) and incubated in microaerobic atmosphere. The identification of species was obtained by the multiplex PCR (mPCR). Altogether 30 isolated strains (13 C. jejuni and 17 C. coli) were subjected to antimicrobial sensitivity testing using the disk diffusion method. Antimicrobial agents on disk and concentrations were as follows: azithromycin (15 μg, Bio-Rad, USA), ciprofloxacin (5 μg, Bio-Rad, USA), enrofloxacin (5 μg, Bayer, Germany), erythromycin (15 IU, Becton Dickinson, UK), gentamicin (10 μg, Bio-Rad, USA), and tetracycline (30 μg, Bio-Rad, USA). From 91 chicken swabs 35 (38.46%) isolates were found mPCR positive, whereas 18 were detected as C. coli and 17 as C. jejuni. Out of 28 laying hen swabs, 14 were Campylobacter spp. positive, and by mPCR 8 (57.14%) were identified as C. coli, and 6 (42.86%) as C. jejuni. The study determined the high frequency of resistant Campylobacter strains (from 46.15 % to 100 %) to all five tested antibiotics. Fluoroquinolones and other antimicrobial agents will continuously be used in human and veterinary medicine, but it should be judiciously and under control, together with routinely susceptibility testing. The relatively high number of campylobacter positive samples points that the approach to the control of campylobacter in Croatia should be changed and that the Action plan, similar to others in European Countries is needed, to assure healthy and safe poultry meat for consumers.

Izvorni jezik
Engleski

Znanstvena područja
Javno zdravstvo i zdravstvena zaštita, Veterinarska medicina

POVEZANOST RADA