Naslov
Ultrastructure and protein pattern of horse-radish (Armoracia lapathifolia Gilib.) crown-gall tissue

Autori
Muraja, Jasmina ; Peškan, Tatjana ; Lorković, Zdravko ; Krsnik-Rasol, Marijana

Izvornik
Acta botanica Croatica (0365-0588) 53 (1994); 1-6

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
crown-gall ; teratoma tissue ; ultrastructures ; protein pattern ; horse-radish

Sažetak
Two horse-radish (Armoracia lapathifolia Gilib.) crown gall tissue lines, one unorganized and the other teratoma producing were compared with the control leaf in ultrastructures and electrophoretic protein patterns. Tumour cdells were similar to unspecialized parenchyma cells. During tumourigenesis the chloroplasts transformed into amyloplasts or leukoplasts. Amylochloroplasts were present in teratoma cells. Large lobed nuclei were observed in both types of transformed cells. Leaf specific polypeptides of about 22-, 21-, 20 and 18 kDa disappeared in tumour extracts and the enzyme Rubisco auantitatively decreased. The polypeptide of about 85 kDa was present only in transformed tissues. In some other proteins only quantitative differences could be detected.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA