Pregled bibliografske jedinice broj: 437785
Organic anion transporting polypeptides (OATP) in zebrafish (Danio rerio): phylogenetic analysis and tissue distribution
Organic anion transporting polypeptides (OATP) in zebrafish (Danio rerio): phylogenetic analysis and tissue distribution // Comparative biochemistry and physiology. Part A, Molecular & integrative physiology, 155 (2010), 3; 327-335 doi:10.1016/j.cbpa.2009.11.011 (međunarodna recenzija, članak, znanstveni)
CROSBI ID: 437785 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Organic anion transporting polypeptides (OATP) in zebrafish (Danio rerio): phylogenetic analysis and tissue distribution
Autori
Popović, Marta ; Žaja, Roko ; Smital, Tvrtko
Izvornik
Comparative biochemistry and physiology. Part A, Molecular & integrative physiology (1095-6433) 155
(2010), 3;
327-335
Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni
Ključne riječi
OATP ; zebrafish ; phylogenetic analysis ; membrane topology ; tissue distribution
Sažetak
The aim of our study was initial characterization of Organic anion transporting polypeptides (Oatp ; SLC21 gene superfamily) in zebrafish (Danio rerio), as an important model species in biomedical and ecotoxicological research, using phylogenetic analysis, membrane topology prediction and tissue expression profiling. Phylogenetic tree of Oatp superfamily in vertebrates was constructed in Mega Software version 3.1., membrane topology was predicted using HMMTOP algorithm, while qRT-PCR was used to determine tissue specific gene expression levels. Phylogenetic analysis revealed that Oatp superfamily in zebrafish consists of five families that include 14 SLCO genes. Eight out of 14 transporters do have orthologs or co-orthologs in other vertebrates, while 6 members are found only in fish lineage. Topology analysis showed that all zebrafish Oatps consist of 12 transmembrane domains with the large fifth extracellular loop (LP5). Tissue distribution analysis revealed that the expression patterns of Oatp2a1, Oatp2b1 and Oatp3a1 follows tissue distribution patterns of their mammalian (co)orthologs. Expression pattern of a newly identified Oatp1d1 is similar to mouse Oatp1a4, while other new zebrafish Oatps (Oatp1e1, 1f2 and 1f4) do not resemble any of the mammalian Oatps. In summary, the described comprehensive analysis of Oatp superfamily in fish represents the first research step needed for understanding of ecotoxicological relevance of uptake transporters in aquatic organisms.
Izvorni jezik
Engleski
Znanstvena područja
Geologija, Biologija
POVEZANOST RADA
Projekti:
098-0982934-2745 - Ekotoksikološko značenje ABC transportnih proteina u vodenih organizama (Smital, Tvrtko, MZOS ) ( CroRIS)
Ustanove:
Institut "Ruđer Bošković", Zagreb
Citiraj ovu publikaciju:
Časopis indeksira:
- Current Contents Connect (CCC)
- Web of Science Core Collection (WoSCC)
- Science Citation Index Expanded (SCI-EXP)
- SCI-EXP, SSCI i/ili A&HCI
- Scopus
- MEDLINE
