Naslov
Preliminary investigations on relationship between polymorphism at CAST locus and the quality of pork

Autori
Đurkin, Ivona ; Margeta, Vladimir ; Margeta, Polona ; Kralik Gordana ; Kušec, Goran

Izvornik
Poljoprivreda (Osijek) (1330-7142) 15 (2009), 2; 53-58

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, prethodno priopćenje, znanstveni

Ključne riječi
CAST gene ; polymorphism ; pig ; meat quality

Sažetak
The present study was performed in order to investigate a relationship between polymorphisms on the calpastatin gene (CAST) and pig meat quality traits. Investigation was carried out on 29 gilts and barrows, crosses of Large White x German Landrace randomly selected at slaughter line. Pigs were slaughtered at 130-150 kg of live weight and blood samples for genomic DNA analysis were taken. The following indicators of meat quality and meat chemical composition were evaluated: pH and electric conductivity measured 45 minutes post mortem in m. Longissimus dorsi (LD muscle) and in M. Semimembranosus (SM muscle) ; electric conductivity measured at the same locations after 24h of cooling ; drip loss (determined by “bag method”) ; colour (measured with Minolta chromameter and expressed as Hunter L, a, b values) ; instrumental tenderness assessed as Warner-Bratzler (WB) shear force and moisture, fat, protein and collagen content (%) determined on cooked LD muscle after 24h of thawing. The amplification products of the CAST gene were digested with HinfI restriction endonuclease and three genotypes (AA, BB and AB) were revealed. Statistical analysis showed that meat originating from pigs of AB genotype had the lowest WB shear force and the highest protein content of cooked LD muscle. As for the indicators of technological meat quality, statistically significant differences (p<0.05) were found between genotype AA and both BB and AB genotype for electric conductivity measured in LD muscle after 24h of cooling, as well as between BB and both AA and AB genotypes for drip loss.

Izvorni jezik
Engleski

Znanstvena područja
Poljoprivreda (agronomija)

POVEZANOST RADA