Naslov
Interaction Between Listeria monocytogenes and Acanthamoeba castellanii

Autori
Mihelcic, Mirna ; Cujec, Ivana ; Bacun-Druzina, Visnja ; Abram, Maja

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Acta Microbiologica et Immunologica Hungarica / Nagy, K ; Marialigeti, K. - Budimpešta : Akadémiai Kiadó, 2009, 205-205

Skup
Second Central European Forum for Microbiology

Mjesto i datum
Keszthely, Mađarska, 07.10.2009. - 09.10.2009

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Listeria monocytogenes; free-living protozoa; Acanthamoeba castellanii

Sažetak
Listeria monocytogenes, gram positive, facultative intracellular rod, is found ubiquitously in the environment and is capable to grow and survive in a wide range of environmental conditions. However, the current knowledge of its ecology and in particular, the mode of its environmental survival and transmission remains limited. It has been suggested that free-living protozoa serve as an environmental reservoirs and vehicles for the transmission of several obligate and facultative intracellular pathogens, but the ability for intracellular multiplication and survival of L. monocytogenes in Acanthamoeba spp. is still unclear. The aim of this study was to investigate the interaction between the protozoan Acanthamoeba castellanii (ATCC 30234) and EGD strain of Listeria monocytogenes. The results indicate that L. monocytogenes does not reside within A. castellanii, as we were unable to recover any intracellular bacteria after 4 hours of co-cultivation. However, extracellular bacteria benefit from co-cultivation with amoeba showing increased growth and multiplication. In conclusion, Listeria monocytogenes is not able to survive inside Acanthamoeba castellani, but is quickly degraded under the laboratory conditions tested. A. castellani is not able to act as environmental reservoir for L. monocytogenes but may provide a survival advantage for extracellular listeria during co-cultivation of the two organisms.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

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