Naslov
Application of glucose oxidase biosensor with glucose oxidase immobilised in different photocurable polymeric membranes for glucose determination in alchocolic beverages

Autori
Ninčević Grassino, Antonela, Milardović, Stjepan, Grabarić, Zorana, Grabarić, Božidar S.

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Abstract book of 15th World Congress of Food Science and Technology / Sigge, G. - Cape Town : SAAFoST, 2010

Skup
15th World Congress of Food Science and Technology, IUFoST 2010

Mjesto i datum
Cape Town, Južnoafrička Republika, 22.08.2010. - 26.08.2010

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
glucose oxidase biosensor; photocurable membranes; mixed solvents; alcoholic beverages

Sažetak
Introduction: A large number of papers describe the development of organic phase enzyme electrodes as promising tools in biocatalytic processes, environmental monitoring, and drug and food analysis. The objective of this work was to investigate the use of glucose oxidase biosensor with glucose oxidase immobilized in different photocurable polymeric membranes for determination of analytes in mixed aqueous buffer/organic solvents. Methodology. The glucose oxidase was immobilized in photopolymer (polyacrylamide, polyvinylpyrolidon or their mixture) on the surface of the indium(III) and iron(III) hexacyanoruthenate film, electrodeposited on graphite electrode. Voltammetric and amperometric measurements were performed with a polarographic analyzer, under batch conditions. Results and Discussion. The response of biosensor was measured as a function of selected organic solvents containing 0.1 M phosphate buffer solution (pH = 6.86) at -100 mV against a reference Hg/Hg2Cl2/3 M KCl electrode. It was found that maximum enzyme activity was obtained with addition of 10 % of methanol. The increases of volume fractions of methanol (from 20 to 40 %) as well as ethanol (from 10 to 40 %) decreased the biosensor response. Addition of isopropanol, even at small volume fractions (10 %), decomposes the photopolymeric membrane. Therefore, the determination of glucose in commercial alcoholic beverages has been performed in phosphate buffer and phosphate buffer/methanol (φ = 10 %) mixture. The linear range was obtained from 0.1 to 0.8 mM (r2 = 0.9925) for mixed solvent, as well as phosphate buffer (r2 = 0.9996). Conclusions. The fast and easy preparation of the biosensor and its long life, if glucose oxidase was immobilised in mixed polymeric photocurable membrane, as well as reasonably accurate glucose determination, make the proposed procedure appropriate for quality control of the alcoholic beverages in water or in mixed solvents.

Izvorni jezik
Engleski

Znanstvena područja
Kemija

POVEZANOST RADA