Naslov
Galectin-3, a β -galactoside binding lectin affects cytokine secretion by human macrophages

Autori
Novak, Ruđer ; Dabelić, Sanja ; Lepur, Adriana ; Dumić, Jerka

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Cytokine+, Special Issue - Abstracts and Reviews / Duff W, Gordon ; Durum K, Scott - Lisabon : Elsevier, 2009, 103-103

Skup
Tri-Society Annual Conference 2009 of the Society for Leukocyte Biology, International Cytokine Society, & International Society for Interferon and Cytokine Research

Mjesto i datum
Lisabon, Portugal, 18.10.2009. - 21.10.2009

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
macrophages; cytokines; galectin-3

Sažetak
Galectin-3, a β -galactoside binding lectin exerts important roles in many biological processes (adhesion, proliferation, differentiation, apoptosis) thus being involved in numerous (patho)physiological processes, such as immune reactions, neoplastic transformation, spreading metastases. Being one of the key lectins of innate and acquired immunity, galectin-3 is considered a powerful pro-inflammatory signal, e.g. it triggers/promotes respiratory burst in monocytes, acts as a monocyte/macrophage chemoattractant and promotes survival of inflammatory cells through its anti-apoptotic activity. The aim of this study was to ascertain the role of galectin-3 in physiology of macrophages by measurement of cytokine secretion by differentiated human monocytes (M1 and M2a/c macrophages) after cell exposure to the recombinant human galectin-3 (rhGal-3). Human PBMCs were isolated using Ficoll-Paque and monocytes were separated by surface adhesion to the culture plates in RPMI. To differentiate into: M1 macrophage type monocytes were treated by M-CSF (20 ng/ml) for 7 days ; for M1 polarization LPS (1 mg/ml) and IFNγ (5 ng/ml) were added in the last 24 hours ; M2a/c macrophage type monocytes were treated with GM-CSF (100 ng/ml) for 7 days ; for M2a/M2c polarization IL-4 (20 ng/ml)/IL-10 (10 ng/ml) were added in the last 48 hours. After differentiation cells were treated with rhGal-3 (1µ M) for the next 24 hours. Flow cytometry was applied to determine inflammatory cytokine secretion using capture beads and also to confirm the expression of specific surface markers. Dead cells were excluded as 7AAD or PI positive. M1 polarization was confirmed by elevated TNF-α , IL-1ß and IL-6 levels and low surface CD206 expression in respect to the M2 type. Interestingly, secretion of IL-8 was significantly elevated by M2a/c in respect to M1 macrophages, thus IL-8 could be a novel marker of M1 vs. M2a/c macrophage differentiation. M1 macrophages showed no cytokine response to galectin-3, while M2 macrophages had markedly different secretion patterns after treatment with rhGal-3: IL-6 and IL-8 were elevated in M2a, while TNF-α , IL-6 and IL-8 were elevated in M2c macrophages. Given the importance of galectin-3 in monocyte/macrophage physiology, further experiments will be undertaken to elucidate mechanisms by which galectin-3 regulates secretion of target cytokines in different types of macrophages.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA