Naslov
Title: Molecular characterization of the Dot/Icm-translocated AnkH and AnkJ eukaryotic-like effectors of Legionella pneumophila

Autori
Habyarimana, Fabien ; Al Khodor, Souhaila ; Šantić, Marina ; Price, Chris ; Abu Kwaik, Yousef

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Skup
Legionella 2009

Mjesto i datum
Pariz, Francuska, 13.10.2009. - 17.10.2009

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
legionella; ANK repeat; Dot/Icm; effector

Sažetak
The eukaryotic-like Ankyrin H (AnkH) and AnkJ proteins of Legionella pneumophila are required for intracellular proliferation. In this report, we show that expression of ankH and ankJ is temporally triggered by a network of interacting regulatory cascades. Intracellular bacteria translocate AnkH and AnkJ and 5 other Ank proteins into the macrophage cytosol via the Dot/Icm type IV secretion system. The IcmSW chaperones are essential for translocation of AnkJ but not AnkH. The ankH and ankJ mutants are severely defective in intrapulmonary proliferation in mice. The 10 C-terminal residues and the ANK domains of AnkH and AnkJ are required for translocation. Expression of AnkH and AnkJ fusions within HEK293 cells show a punctuate distribution in the cytosol but no association with endocytic vesicles, the Golgi apparatus or the ER. Interestingly, the defect in intracellular proliferation of the ankH or ankJ mutants is rescued in HEK293 cells expressing the respective effector. W e conclude that AnkH and AnkJ are effectors translocated by the Dot/Icm system by distinct mechanisms and modulate distinct cytosolic processes in the host cell. This is the first demonstration of a trans-complementation of an effector mutant of L. pneumophila through expression of the respective effector in the host cell.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti, Kliničke medicinske znanosti

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