Pregled bibliografske jedinice broj: 431860
Assaying the carotenoid content in corn and sweet potato flours by photoacoustic spectroscopy
Assaying the carotenoid content in corn and sweet potato flours by photoacoustic spectroscopy // 6th International Conference "Instrumental Methods of Analysis - Modern Trends and Applications", 4- 8. listopad 2009 ; Book of abstracts / Efstathiou, Constantinos ; Calokerinos, Antony ; Ochsenkuehn-Petropoulou, Maria (ur.).
Atena: National and Kapodistrian University of Athens ; National Technical University of Athens, 2009. str. 277-277 (poster, međunarodna recenzija, sažetak, znanstveni)
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Naslov
Assaying the carotenoid content in corn and sweet potato flours by photoacoustic spectroscopy
Autori
Luterotti, Svjetlana ; Kljak, Kristina ; Grbeša, Darko ; Bicanic, Dane ; San Martin, Eduardo ; Spruyt, Ruud
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni
Izvornik
6th International Conference "Instrumental Methods of Analysis - Modern Trends and Applications", 4- 8. listopad 2009 ; Book of abstracts
/ Efstathiou, Constantinos ; Calokerinos, Antony ; Ochsenkuehn-Petropoulou, Maria - Atena : National and Kapodistrian University of Athens ; National Technical University of Athens, 2009, 277-277
Skup
4-8. listopad 2009
Mjesto i datum
Atena, Grčka, 04.10.2009. - 08.10.2009
Vrsta sudjelovanja
Poster
Vrsta recenzije
Međunarodna recenzija
Ključne riječi
total carotenoids assay; corn flours; sweet potato flours; photoacoustic spectroscopy
Sažetak
This is the first attempt to use photoacoustic spectroscopy (PAS) for quantification of carotenoids in powder-like samples, namely in corn and sweet potato flours. In this study spectrophotometry has been used as an established absolute method. PAS measurements were performed at room temperature at 488 nm of the argon laser (f=9 or 30 Hz, tc=1 s, incident laser power 70-74 mW). Central semispherical cavity of the PAS cell (diameter 8 mm accommodated flour specimen ; this latter was loaded flush with the surface of the plate using a spatula. PAS measurements were repeated within a run and within several days or months. Thirty-three corn flours were investigated ; linear analytical response was obtained for the samples containing 0.1-26 ppm total carotenoids(R=0.91-0.92), for upto 36 ppm TC power function was obtained (R=0.95-0.97). DL values obtained at 9 and 30 Hz were 2.14 and 6.51 ppm TC. Nine sweet potato flours were also analyzed. Two linear regions were established: upto 18 ppm beta carotene (R=0.92-0.96) and from 40-240 ppm (R=0.94-0.95). Respective DL values were 0.70 and 0.90, namely 12.43 and 19.86 ppm beta-carotene. Comparable repeatability (n=3-12) and intermediate precision (n=6-28) were evaluated for both frequencies: 0.1-17.1% and 5.3-14.7% for corn flours and 1.4-9.1% and 4.2-23.0% for sweet potato flours, resp. Our results show that PAS might be successfully used as an analytical tool for quick and simple screening of flour samples for their nutritional value through the content of carotenoids.
Izvorni jezik
Engleski
Znanstvena područja
Fizika, Kemija, Poljoprivreda (agronomija)
POVEZANOST RADA
Projekti:
006-0061117-1243 - Interakcije galija(III) i željeza(III) s antiproliferativnim lijekovima (Kujundžić, Nikola, MZOS ) ( CroRIS)
178-1780496-0368 - Hranidbena, antioksidativna i prebiotička svojstva kukuruza za domaće životinje (Grbeša, Darko, MZOS ) ( CroRIS)
Ustanove:
Farmaceutsko-biokemijski fakultet, Zagreb,
Agronomski fakultet, Zagreb
