Naslov
Development of anther-derived flue-cured tobacco dihaploids from PVY resistant DH10 hybrid

Autori
Šmalcelj, Blažica ; Ćurković Perica, Mirna

Izvornik
Die Bodenkultur (0006-5471) 51 (2000), 1; 11-17

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Nicotiana tabacum; dihaploids; PVY resistance; yield; quality

Sažetak
Damage caused by potato virus Y (PVY) has motivated a breeding program which aimed at the production of a flue-cured tobacco cultivar resistant to PVY, with better yield and quality than standard cultivars grown in Croatia. Anthers were collected from the commercially grown hybrid DH10, a cross of line GV3 and cv. Virginia D, with favourable agronomic traits. Dihaplods were raised from the midvein explants of anther derived haploids. Forty-seven dihaploids raised in this experiment were evaluated in comparative field trails, over a 3-year period. Three out of 47 tested dihaploid lines equaled agronomic and exceeded commercial properties of DH10 hybrid. Their yield and PVY- resistance were similar to that of the DH10 hybrid, but the price ($/kg)was considerably higher. These results show that dihaploid tobacco lines do not necessarily exibit yield reduction and that anther culture followed by diploidization is a useful method to accelerate the development of new, improved, homozygous tobacco varieties. Significant differences between dihaploids derived from the same haploid plant were observed in flowering date, PVY resistance, plant height, number of leaves, leaves width and length, yield and price, showing that dihaploid lines derived from the same haploid individual can differ among themselves as well as those derived from different haploids.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

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