Naslov
Prognostic value of microphthalmia-associated transcription factor as a marker for the detection of circulating melanoma cells in patients with melanoma

Autori
Šamija, Ivan ; Lukač, Josip ; Marić-Brozić, Jasmina ; Šitum, Mirna ; Kusić, Zvonko

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
2009 Annual Meeting of the Croatian Immunological Society Book of Abstracts / - Zagreb : Hrvatsko imunološko društvo, 2009, 31-31

Skup
2009 Annual Meeting of the Croatian Immunological Society

Mjesto i datum
Starigrad, Hrvatska, 01.10.2009. - 04.10.2009

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Domaća recenzija

Ključne riječi
melanoma; microphthalmia-associated transcription factor; circulating tumor cells; reverse-transcription polymerase chain reaction

Sažetak
Microphthalmia-associated transcription factor (MITF) was investigated in this study as a marker for the detection of circulating melanoma cells by reverse transcription polymerase chain reaction (RT-PCR).The aim of the study was to determine the prognostic value of MITF in patients with melanoma. Peripheral blood samples from 201 melanoma patients in all stages of the disease were analyzed by RT-PCR for the expression of MITF. A blood sample for analysis was obtained from each patient at the time of inclusion in the study. MITF mRNA was detected in blood samples from 32 (15.9%) out of 201 melanoma patients. Median follow-up for patients was 53 months (range 3-84 months). Both progression-free survival (p=0.005, log-rank test) and overall survival (p=0.042, log-rank test) were significantly shorter in patients positive for MITF in comparison to patients negative for MITF. In a multivariate regression analysis the value of MITF was an independent prognostic factor for progression-free survival (hazard ratio=2.54 ; 95% CI, 1.06-6.13 ; p=0.038) together with AJCC stage of the disease, while the AJCC stage of the disease was the only independent prognostic factor for overall survival. Our results suggest that analysis MITF as a marker for the detection of circulating melanoma cells has a prognostic value in patients with melanoma.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Temeljne medicinske znanosti, Kliničke medicinske znanosti

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