Naslov
Single electroconvulsive shock does not influence COX-2 and HSP70 expressions in the rat frontal cortex and hippocampus

Autori
Hrelja, Ana ; Pilipović, Kristina ; Župan, Gordana

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Abstracts of the 3rd Croatian Congress of Neuroscience ; PP71 / - , 2009, 78-78

Skup
3rd Croatian Congress of Neuroscience

Mjesto i datum
Zadar, Hrvatska, 24.09.2009. - 26.09.2009

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
COX-2; HSP70; electroconvulsive shock; rat

Sažetak
Introduction: Electroconvulsive therapy is commonly used in psychiatry as a treatment for pharmacotherapy-resistant cases of severe depression and other affective disorders but there are still many questions concerning the mechanisms underlying its therapeutic and side effects. The aim of our study was to investigate whether single electroconvulsive shock (ECS)affects expressions of COX-2 inflammatory and heat shock HSP70 proteins in the rat frontal cortex and hippocampus. Materials & Methods: Experiments were performed on male Wistar rats (age 2-3 months ; weight 230-280 g). Animals were randomly divided into five experimental groups. Rats of the control group were subjected to the sham ECS procedure. Other groups of animals received single ECS (100 mA, 50 Hz, 0.4 ms interval, 400 ms total duration) via earclip electrodes using an ECS apparatus (Ugo Basile, Italy). Animals were decapitated at different time points (0.5 h, 3 h, 12 h, 24 h) during the first 24 h after single sham ECS procedure or ECS-induced seizures. Western blotting analyses of COX-2 and HSP70 expressions were performed in the frontal cortex and hippocampus. Results: Cortical and hippocampal COX-2 and HSP70 expressions in rats with ECS- induces seizures were not significantly changed in comparison to the values registered in the sham- ECS treated rats, at any of the time points examined. Conclusion: Our findings suggest that single ECS does not affect COX-2 and HSP70 expressions in the rat frontal cortex and hippocampus, at different time points within the first 24 h after its application.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

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