Naslov
Virus attenuation after deletion of the cytomegalovirus Fc receptor gene is not due to antibody control

Autori
Crnković-Mertens, Irena ; Messerle, Martin ; Milotić, Irena ; Szepan, Uwe ; Kučić, Natalia ; Krmpotić, Astrid ; Jonjić, Stipan ; Koszinowski, Ulrich H.

Izvornik
Journal of virology (0022-538X) 72 (1998), 2; 1377-1382

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
cytomegalovirus ; Fc receptor ; imunoevasive function of MCMV-FcR

Sažetak
The murine cytomegalovirus (MCMV) fcr-1 gene codes for a glycoprotein located at the surface of infected cells which strongly binds the Fc fragment of murine immunoglobulin G. In order to determine the biological significance of the fcr-1 gene during viral infection, we constructed MCMV fcr-1 deletion mutants and revertants. The fcr-1 gene was disrupted by insertion of the E. coli lacZ gene. In another mutant also the marker gene was deleted by recombinase cre. As expected for its hypothetical role in immunoevasion, the infection of mice with fcr-1 deletion mutants resulted in significantly restricted replication in comparison with wild-type MCMV and revertant virus. In mutant mice lacking antibodies, however, the fcr-1 deletion mutants replicated also poorly. This demonstrated that the cell surface expressed viral glycoprotein with FcR activity strongly modulates the virus-host interaction but this biological function is not caused by the immunoglobulin binding property.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA