Naslov
Determining the population structure of Citrus tristeza virus - how many clones should be sequenced?

Autori
Černi, Silvija ; Ruščić, Jelena ; Šatović, Zlatko ; Krajačić, Mladen ; Škorić, Dijana

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
10. hrvatski biološki kongres, zbornik sažeteka / Besendorfer, Kopjar, Vidaković-Cifrek, Tkalec, Bauer, Lukša - Zagreb : Hrvatsko biološko društvo, 2009, 109-110

ISBN
978-953-6241-07-1

Skup
10. hrvatski biološki kongres

Mjesto i datum
Osijek, Hrvatska, 14.09.2009. - 20.09.2009

Vrsta sudjelovanja
Poster

Vrsta recenzije
Nije recenziran

Ključne riječi
CTV; quasispecies; pathotype

Sažetak
According to quasispecies theory, the populations of RNA viruses display high genetic diversity. These dynamic populations are composed of a swarm of genomic variants that coexist within individual isolates. Genetic variants found within isolates of Citrus tristeza virus (CTV), the most devastating citrus pathogen, may be of different pathotype (have different pathogenic potential). Bearing in mind that minor population variants might influence symptom expression, and that the selection of variants during natural virus transmission is random, the information about virus population structure is of great epidemiological importance. The most reliable method used for the characterization of virus variants encompasses separation of individual variants by cloning, their identification and quantification by SSCP (single-strand conformation polymorphism) analysis and sequencing. However, in order to determine the virus population structure, authors sequence different number of clones, often only a few per isolate. The aim of this study was to evaluate whether sequencing greater number of clones results in better information about CTV pathotypes present in the population. Different pathotypes were detected by successive analysis of coat protein gene sequences of eight CTV isolates displaying different genetic diversity. Results suggest that, depending on a genetic diversity of the isolate, it is advisable to analyze up to 30 clones per sample, which is more than scientist usually do.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

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