Naslov
Modulation of peripheral blood lymphocyte subpopulations in patients with severe brain injury

Autori
Sotošek Tokmadžić, Vlatka ; Laškarin, Gordana ; Mahmutefendić, Hana ; Lučin, Pero ; Rukavina, Daniel ; Šustić, Alan

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Abstracts of the 22nd Annual Congress of European Society of Intensive Care Medicine ; u: Journal of Intensive Care Medicine / - , 2009

Skup
Annual Congress of European Society of Intensive Care Medicine (22 ; 2009)

Mjesto i datum
Beč, Austrija, 11.10.2009. - 14.10.2009

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
severe brain injury; immunity; T lymphocytes; NK cells; NKT cells

Sažetak
Nosocomial infections are leading causes of increased morbidity and mortality of severe brain injured patients1. The mechanism underlying the susceptibility to the infections is a subject of great scientific interest and still to be clarified2. It has been recently recognized that injury of brain induces a disturbance of balance between the central nervous and immune system3. The aim of this study was to investigate changes in frequency of lymphocytes subpopulation in peripheral blood of patients with severe brain injury during the course of intensive care treatment. Human peripheral blood samples were taken from the severe brain-injured patients at day 1, 4 and 7 and peripheral blood mononuclear cells (PBMC) were immediately isolated by gradient density centrifugation. The percentage of lymphocytes subpopulation were analyzed by simultaneous detection of surface antigens using fluorochrome conjugated monoclonal antibodies directed toward CD3, CD56, CD16, CD4, CD8, CD5, CD19. T lymphocytes were distinguished from the other lymphocyte subpopulation as cells labeled with anti-CD3 monoclonal antibody but negative for CD56 staining (CD3+CD56-). T cells subsets were determined as: CD3+CD56-CD4+ (helper T cells) and CD3+CD56-CD8+ (cytotoxic T cells). The cells stained as CD3-CD56+ and CD3+CD56+ were determined as NK or NKT cells respectively. B lymphocytes were stained for CD5+CD19+. The frequency of CD4+, CD8+, CD16+ and CD56+ within entire T cell population was analyzed as well. Results: At day 4 after the injury the percentage of T lymphocytes significantly diminished and their number restored at day 7. It is a consequence of CD3+CD56-CD8+ fluctuation rather than changes of CD3+CD56-CD4+ subset. The frequency of NK and NKT cells showed the similar time dependent pattern whereas the percentage of B cells did not change basically. The frequency of CD4 expressing T cells did not significantly change whereas CD8 expressing T cells decreased at day 4 and restored at day 7 to the initial level. The decrease of cells with cytotoxic phenotype (CD3+CD56-CD8+ T cells, CD3-CD56+ NK cells, CD3+CD56+ NKT cells) might explain high incidence of susceptibility to infection of patients with severe brain injury

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti

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