Naslov
The consequencs of the in vivo depletion of CD4 and/or CD8+ lymphocytes on regenerative liver growth

Autori
Ćuk, Mira ; Petković, Marija ; Trobonjača, Zlatko ; Milin, Čedomila ; Dvornik, Štefica ; Ravlić-Gulan, Jagoda ; Mrakovčić-Šutić, Ines ; Radošević-Stašić, Biserka ; Rukavina, Daniel

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Annual meeting of the Croatian Immunological Society, Periodicum Biologorum 99 (1997), suppl. 2 / Vitale, Branko - Zagreb : Hrvatsko prirodoslovno društvo, 1997, 23-23

Skup
Annual meeting of the Croatian Immunological Society

Mjesto i datum
Zagreb, Hrvatska, 06.11.1997. - 07.11.1997

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
liver regeneration; depletion od CD4 and/or CD8 + T lymphocytes; mice

Sažetak
Liver regeneration after the loss of hepatic tissue or injury is regulated by complex network of different growth factors and cytokines like hepatocyte growth factor, tumor necrosis factor-a, IL-6, epidermal growth factor and transforming growth factor-a. Some of them are liver derived while the others have extrahepatic origin and might be linked with the function of lymphatic tissue. In these events particular role may have activated Kuppfer cells (or other types of nonparenchimal liver cells), lymphocytes with morphogenetic capabilities and the cells of innate immunity. Since the role of T cell subpopulation in the control of liver regeneration is not often emphasized in this work we attempted to analyze the consequences of in vivo depletion of T-lymphocyte subsets on hepatic DNA, RNA, and proteins, as well as, on serum composition of partially hepatectomized (pHx) mice and vice versa the effects of pHx on the spontaneous recovery of lymphatic tissue after the selective use of monoclonal antibodies. In vivo depletion of CD4+, CD8+ or both T lymphocytes subsets was accomplished by the treatment of mice with rat anti-murine CD4 (GK 1.5 ; IgG2b) and rat anti-mouse CD8 (YTS 169.2 ; IgG2b) monoclonal antibodies (mAbs), respectively. Estimation of liver growth and hepatic intermediate metabolites in serum was made using the standard methods. Isolation of spleen cell and surface phenotype determination was made using the FACScan analysis. The results showed that in vivo depletion of CD8+ and CD4+CD8+ lymphocytes prior to pHx significantly augment the content of RNA and proteins in early phase of regenerating liver. Removal of CD4+CD8+ cells translocated the peak of protein synthesis from 2nd to1st post Hx day. On the other hand, pHx stimulated the recovery of CD4+ cells in CD4 depleted mice, decreased the compensatory hyperplasia of CD4+ cells in CD8+ depleted mice and augmented the proportion of CD4+ cells in CD4+CD8+ depleted mice. Depletion of CD4+ lymphocytes augmented and depletion of CD4+CD8+ lymphocytes decreased the concentration of creatinin in pHx mice. An increase of urea level was seen after treatment with anti CD8 mAb. The concentrations of other serum constituents (total proteins, glucose, calcium and phosphate) in pHx mice depleted of CD4+ and or CD8+ T lymphocytes were found to be unchanged. The data point to the importance of lymphatic system in the control of liver growth.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti

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