Naslov
Effect of Growth Phase on the Content and Composition of Ceramides of the Hydrocarbon-assimilating Yeast Candida lipolytica

Autori
Rupčić, Jasminka ; Milin, Čedomila ; Marić, Vladimir

Izvornik
Systematic and Applied Microbiology (0723-2020) 22 (1999), 3; 486-491

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
yeast; Candida lipolytica; sphingolipids; ceramides; growth phase; fatty acids; phytosphingosines

Sažetak
Candida lipolytica yeast was grown batchwise on n-hexadecane as the carbon and energy source. Ceramides were quantitatively isolated from total lipids of exponential and stationary phase cells by a combination of column chromatography and preparative high-performance thin-layer chromatography. After acid methanolysis their composition was analyzed by gas-liquid chromatography. The ceramide content of the exponential phase cells was two times higher than the one of the stationary phase cells. The composition of long-chain base moiety of ceramides did not change significantly during the growth. In both growth phases 19-phytosphingosine was the major long-chain base. However, the fatty acid composition of ceramides changed greatly during growth. In the exponential growth phase, ceramides contained predominantly fatty acids greater than 20 carbon atoms, while fatty acids shorter than 20 atoms predominated in ceramides of the stationary phase, 16 : 0 being the main one. In the exponential growth phase fatty acid moiety of ceramides was characterized by unusually high degree of unsaturation and relatively high proportion of odd-numbered fatty acids. However, the proportion of both, unsaturated and odd-numbered fatty acid decreased significantly in ceramides of the stationary phase. The unexpected finding was the absence of fatty acid hydroxylation of ceramides in the exponential phase cells and unusually low degree of hydroxylation in the stationary phase.

Izvorni jezik
Engleski

Znanstvena područja
Farmacija, Prehrambena tehnologija

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