Naslov
Genetic and protein changes of E-cadherin in meningiomas

Autori
Pećina-Šlaus, Nives ; Nikuševa Martić, Tamara ; Zeljko, Martina ; Deak, Adam Jakov ; Tomas, Davor

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Abstracts of The EMBO meeting advancing the life sciences / - Amsterdam, 2009, 80-81

Skup
The EMBO meeting advancing the life sciences

Mjesto i datum
Amsterdam, Nizozemska, 29.01.2009

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
CDH1; E-cadherin; immunostaining; loss of heterozygosity; meningiomas; replication error

Sažetak
The molecular mechanisms and candidate genes involved in development of meningiomas still need investigation and elucidation. In the present study thirty-three meningiomas were analyzed regarding genetic changes of tumor suppressor gene E-cadherin (CDH1), a component of adherens junction and an indirect modulator of the wnt signaling. Gene instability was tested by polymerase chain reaction/loss of heterozygosity (LOH) method. Protein expression was analyzed by immunohistochemistry. The results of our analysis showed altogether 32% of samples with LOH of the CDH1 gene. Interestingly, another type of genomic instability was detected &#190; ; replication error-positive samples (RER+). Three out of 28 heterozygous samples were RER-positive (11%). The instability is the result of impaired cellular mismatch repair. Fibrous and angiomatous cases showed higher percent of genetic changes, 67% and 75%, respecitively. Immunostaining showed that 58% of samples had downregulation of E-cadherin expression. Five out of nine samples with LOH were accompanied with the downregulation of E-cadherin protein expression (56%). One RER+ sample had lower expression of E-cadherin. We noticed that 37% of samples with lower E-cadherin expression had beta-catenin located in the nucleus. Also, 75% of samples with genomic instabilities had beta-catenin in the nucleus. Our findings demonstrated that there is significant association between the genetic changes of CDH1 and the nuclear localization of beta-catenin protein (c2 =5, 25, df =1, P<0, 022). Our results suggest that genetic instabilities of the E-cadherin gene have a role in meningioma development and progression. Detected microsatellite instability indicates that mismatch repair may also be targeted in meningioma.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA