Naslov
Stress exerts tissue-specific effects on sialyltransferase activity

Autori
Dabelić, Sanja ; Lauc, Gordan ; Breen, Kieran ; Flögel, Mirna

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, neobjavljeni rad, znanstveni

Izvornik
Italian Biochemical Society Transaction Vol. 15 / D'Alessio i sur. - Napulj : Societa Italiana di Biochimica e Biologia Molecolare, 2000, 267-267

Skup
Societa Italiana di Biochimica e Biologia Molecolare 45 Congresso Nazionale

Mjesto i datum
Napulj, Italija, 20.09.2000. - 23.09.2000

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
stress; glycosyltransferases; sialyltransferase

Sažetak
INTRODUCTION: Metabolic response to psychological stress is a very complex and demanding physiological process that involves numerous organs and organ systems. Though it is a highly important for survival in ever changing environment, its excessive activation is associated with various detrimental effects. A number of epidemiological and experimental studies clearly demonstrated a link between stress and the development and course of many diseases from simple virus infections and gastric ulcers to cardiovascular diseases and cancer. Previously we have shown specific changes in protein glycosylation and expression of lectins that occur during stress response. Here we report that stress also affects activity of sialyltransferases (STs). MATERIALS AND METHODS: Using asialofetuin as an acceptor and 14C-CMP-NeuAc as a donor of sialic acid we have measured total ST activity in rat liver, kidney, heart, skeletal muscle, adrenal gland, and medulla spinalis. Rats were exposed to immobilization stress, either acutely (one 2 h episode), or chronically (multiple immobilization, swim, and footshock stress for eight days), and the activity of STs in their tissues was compared to ST activity in tissues of control rats. RESULTS: Despite the fact that all analyzed tissues were exposed to same hormonal signals, the impact of these signals on ST activity varied significantly (Fig 1.). In liver and spleen acute stress caused approximately 40% increase in ST activity. However, when the exposure to stressors was prolonged, their effects in liver and spleen were opposite. While ST activity in spleen nearly doubled (193% of control), in liver it decreased to 82% of the activity in control rats. Beside in liver and spleen, ST activity in other analyzed non-neural tissues did not appear to be significantly influenced by stress. In neural tissues there was generally a reduction of ST activity as a consequence of stress. The only exception was the medulla spinalis where we did not observe any changes. Interestingly, despite significantly changed ST activity in some tissues, in the same tissues we did not observe any changes in sialoglycoprotein patterns.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA