Naslov
Age-dependent effect of peptidoglycan monomer linked with zinc on the generation of suppressor macrophages in mice

Autori
Radošević-Stašić, Biserka ; Ravlić-Gulan, Jagoda ; Trobonjača, Zlatko ; Ćuk, Mira ; Muhvić, Damir ; Milin, Čedomila ; Rukavina, Daniel

Izvornik
Croatian medical journal (0353-9504) 38 (1997), 3; 212-216

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
aging; concanavalin A; PGM-Zn; suppressive macrophages; mice

Sažetak
Aim: To quantify the influence of age of mice treated with peptidoglycan-monomer linked with Zn (PGM-Zn) upon the generation of peritoneal exudate cells (PEC) with suppressive activity. Method: BALB/c mice, aged 10-12 or 2, 5-3, 5 months were treated during 26 days (every 5th day), with one injection of PGM-Zn (10 mg/kg i. p.), or with the same amount of the solvent. One day after the last injection PEC cells were collected and co-cultured with syngeneic old and young splenocytes, stimulated with ConA. Three days later the proliferation of "old" or "young" splenocytes in the presence of "old" or "young" PEC from PGM-Zn or NaCl-treated mice was evaluated.Result: "Old" control splenocytes were less reactive to ConA than those obtained from young animals. Pre-treatment donor mice with PGM-Zn induced in both age groups the appearance of PEC which inhibited proliferative response to ConA in population of both "young" and "old" control splenocytes. However, only "young" PGM-Zn induced PEC exhibited an inhibitory effect on spontaneous proliferation of "old" and "young" control splenocytes and displayed the ability to induce an additional decline of blastogenesis of splenocytes from old PGM-Zn treated mice, pointing to greater suppressive vigour of young macrophages. In contrast to this effect, control PEC, from both young and old NaCl-treated donors gave a stimulating effect on low blastic transformation found in the cultures of "old " and "young" PGM-Zn treated splenocytes. Here again the corrective effect of "young" PEC was greater than the effect of "old" ones. Conclusion: PGM-Zn may induce the appearance of peritoneal macrophages which have suppressive effect on spontaneous and ConA-induced blastogenesis of splenocytes. The effects were, however, dependent on age and dependent on the proliferative activity of responder cells.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA