Naslov
Transfer to in vitro conditions influences expression and intracellular distribution of galectin-3 in murine peritoneal macrophages

Autori
Dumić, Jerka ; Lauc, Gordan ; Hadžija, Mirko ; Flögel, Mirna

Izvornik
Zeitschrift für Naturforschung C (0939-5075) 55 (2000), (3-4); 261-266

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
galectin-3; lectin; macrophages; stress

Sažetak
Galectin-3 is a beta-galactoside-binding lectin that has been implicated in numerous physiological processes, including mRNA splicing, cell differentiation, tumor metastasis and the stress response. We have studied effects of transfer of resident murine peritoneal macrophages to in vitro conditions on galectin-3 in different cell compartments. Galectin-3 was purified by immunoprecipitation with rat monoclonal antibody M3/38, and analyzed by immunoblotting using the same antibody. Transfer to in vitro conditions nearly doubled the total amount of galectin-3 in cells, and caused significant alterations in its intracellular distribution, indicating that galectin-3 is involved in the adaptation of peritoneal macrophages to in vitro conditions.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA