Naslov
Analysis of IAA and its metabolites by immunoaffinity extraction and HPLC-MS/MS

Autori
Rolčik, Jakub ; Penčik, Aleš ; Magnus, Volker ; Salopek-Sondi, Branka ; Strnad, Miroslav

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, ostalo

Izvornik
ACPD 2009, Auxins and Cytokinins in Plant Development, Book of Abstracts / - Prag, 2009, 111-111

Skup
ACPD, Auxins and Cytokinins in Plant Development, International Symposium

Mjesto i datum
Prag, Češka Republika, 10.07.2009. - 14.07.2009

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Immunoaffinity extraction; High-performance liquide chromatography (HPLC); Tandem mass spectrometry (MS/MS); Indole-3-acetic acid (IAA); Auxin conjugates

Sažetak
IAA and its metabolites occur in a very complex matrix of plant samples at diverse concentrations, ranging from tens of nmol to hundreds of fmol per g of fresh weight. To isolate and quantify the metabolites, we developed an analytical protocol combining a highly-specific anti-IAA immunoaffinity extraction with a sensitive and selective LC-MS/MS analysis. By using internal standards for each of the analyzed compounds, we can study IAA and a wide range of its metabolites including amino acid conjugates. The protocol enables to quantify the metabolites in amounts of fresh material as low as 20 mg.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA