Naslov
Membrane phenotype and expression of perforin and serine esterases by CD3- peripheral blood and decidual granular lymphocyte-derived clones

Autori
Gudelj, Lea ; Christmas, Stephen E. ; Laškarin, Gordana ; Johnson, Peter M. ; Podack, Eckhard R. ; Rukavina, Daniel

Izvornik
American journal of reproductive immunology (0271-7352) 38 (1997), 3; 162-167

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
CD3- clones; decidual granular lymphocytes; perforin; peripheral blood lymphocytes; serine esterases

Sažetak
Problem: Human first-trimester pregnancy decidua were found to contain large numbers of perforin (P)-containing cells, which varied in their membrane antigen phenotype. In this study results obtained by analyzing CD3- clones derived from human early pregnancy decidua and peripheral blood are reported. Method of study: Decidual tissue was obtained from vaginal termination of first trimester normal human pregnancies. CD3- clones were generated by limiting dilution cloning, after depletion of CD3+ lymphocytes. Cell membrane phenotype was determined by flow-cytometry. Perforin was detected by fluorescence-activated cell sorter (FACS) analysis of permeabilised cells. Serine esterases (SE) were identified by histochemical staining for BLT-esterase. Results: Cloned decidual cell populations retained the overall antigenic phenotype of freshly isolated decidual natural killer (NK)-like cells. All CD3- clones, either derived from decidua or from peripheral blood contained perforin. Serine esterases were present in every decidual clone analysed. Conclusions: Limiting dilution cloning allows the clear-cut analysis of homogenous subsets of decidua derived NK-like clones. The presence of large amounts of perforin in all of the CD3- clones underlines the extensive transcription of perforin gene by NK-like lymphocytes.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti

POVEZANOST RADA