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After an overnight aerobic incubation at 37 0C typical colonies were picked and identified according to Holt et al. (1994). The strains identified as S. intermedius were routinely tested to some or all of 13 different antimicrobial agents (Table 1).Testing was performed by agar diffusion method using Mueller-Hinton medium and Oxoid's antimicrobial susceptibility test discs. Results and Discussion All tested strains were gram-positive cocci growing in unpigmented colonies. All were (-hemolytic (sheep erythrocytes), catalase-, coagulase- (rabbit plasma) and DNase-positive and oxidase-negative. Acetyl methyl carbinol was not produced, and nitrates were reduced. The antimicrobial susceptibilities of S. intermedius isolates are presented in Table 1. In general, there was no demonstrably significant difference between feline and canine isolates of S. intermedius with respect to their sensitivity to examined antimicrobial agents. However, only a smalţ˙ ˙˙˙˙ ŔFMicrosoft Word 6.0 Document MSWordDocWord.Document.6;ţ˙ ÜĽe3Ŕ e% jjjjjjj”””””” ž ”† 1ŇŇŇŇŇŇŇŇ     7 S ˇ T q juţd#âl number of tested strains were isolated from cats. Table 1. The antimicrobial sensitivity of S. intermedius isolates No. ofSensitiveSkin isolatesOther site's isolatesAntimicrobialstestedn%SRSRstrainsn%n%n%n%Penicillin G401947.51254.51045.5738.91161.1Ampicillin + sulbactam3232100.017100.000.015100.000.0Clavulanate-amoxicillin18717995.714295.374.73797.412.6Cephalothin22620590.717291.0179.03389.2410.8Gentamicin18017999.413797.242.839100.000.0Doxycycline322887.51588.2211.81386.7213.3Minocycline322062.51270.6529.4853.3746.7Chloramphenicol463371.71979.2520.81463.6836.4Erythromycin362261.11470.0630.0850.0850.0Tylosin502448.01551.71448.3838.11361.9Rifampicin3232100.017100.000.015100.000.0Lincomycin321959.41575.0525.0433.3866.7Enrofloxacin20820498.116498.231.84097.612.4S=sensitive, R= resistant In our study the mostćŕęäŤÂăŠăćŕáŒä§•‚äŤÂăŠçćŕęä§Âáä¤Â‚äŤÂăŠîćŕęä§ÂáŒâŁžƒ…‚äŤÂăŠâćŕęä§ÂáäĽÂ‚äŤÂăŠáćŕęŒă˛‰‰Š•áä˘Â‚çŤęŠć悏ç¨ęŠćć‚ŏç˛ęŠçćŠććĂîŠçć‚ŏçŻęQfăç˛ŕŠçćĂîŠç悏ä¤Âę^făçŻôç˛ŕ‚űäľÂęîfăXfăä¤ÂŕôŠäćŕř‚ŏçŹęŠććŠďć‚űä¤ÂęäŤÂăçŹŕř‚űç˛řç¨ęç¨áŠçćƏçŤęçŤáŠç悏çŹęŠďć‚üű‚Ŕ‚Ć‚1ć•çćęä¤Âô•śçęç˛áç˛áŠçćô•íć‚üű‚Ŕ‚Ŕ‚űä ÂëŒćř‚űçŤčŠěćĺç¨ř‚7ć•JĺęŠććô•°çęŠçćô•ŇćęŠçćô•ąçęŒä×ւŏçŻęŠććŠďć‚űçŻëŠáćřç­ęîŠçćƏç­ęŠć悁$fä ÂáŒçÜáäŤÂăçŻŕôäŤÂăçŻŕôç­‚Ŕ‚ä¤ÂęäľÂၠfăáfăŕfăěfăXfăäŤÂăŠáćŕŕôŠĺćŕŕáŠçćŕŕ‚1ć•çćęäŤÂăŠáćŕô•śçęŠçćô•Đçęä¤Âô•Ň䂏ä§ÂꁌgăŠććôŠçćŕ‚ŏçŻęQfăŠçćŕŠçćĂîŠç悏çŹęŠć悏ä¤Âę^făçŻôŠçćŕ‚×ՁXfăä¤Âŕ‚ՏäľÂáŒçŠ‚çŹęŠäć‚ՏäľÂáŒçĽ‚çŹęŠĺć‚ՏäľÂáŒçľ‚çŹęŠăć‚ՏäľÂáŒäľ§‚çŹęŠŕć‚üׂűçŹř‚äĽÂęŘgăä§ÂôŠććôçŹŕ‚űîfăIfăäĽÂŕôĺfăäŤÂăçŹŕŕŕëIfăäŤÂăçŹŕŕĺîfăäĽÂôŠçćŕëŒçÎř$fä ÂáŒçÜáä¤ÂôäĽÂôç­‚üű‚Ŕ‚ç˛ęîŠçć‚ŏçŻęŠććŠçć‚űçŻřçŹęŠçćƏçŹęŠŕ悏ä§ÂꁌgăçŻôçŹŕ‚çŹę§găä§ÂôçŻŕîŠçć‚ŏçŤęçŹÂŠçćĂîŠçć‚űéfăŘgăä§ÂôçŻôçŤŕôä˘Âŕř‚űçŻř‚ä¤ÂęŘgăä§ÂôçŻôçŤîŠäćŕ‚űIfăä¤ÂŕëIfăäŤÂăŠďćŕŕĺîfăä¤ÂôŠçćŕëŒçÎř$fäŤÂăŠďćŕôä¤Âôç­‚Ć‚çŤęçŤáŠçć‚üű‚ŏç˛ęçŤÂçŤîŠçćĂîŠçć‚űç˛éŠĺćřWç•JäęçŻô•Íćęç˛ô•çćęŘgăä§ÂôçŻôç˛ŕô•áćęä§Âô•_çô•ĺćęŠćć‚Ŕ‚çŤęŠç悏ç˛ęŠćć‚üű‚Ŕ‚Ŕ‚űç˛ř‚ŏçŻęŠŕć笂űîfăŘgăä§ÂôŠçćôçŻŕôŠçćŕęŒçÎĺîfăŘgăä§ÂôŠçćôçŻîŠäćŕôŠçćŕęŒçÎř‚ŏç˛ęŠçćŠĺćÊä悏ä¤ÂęŘgăä§ÂôŠçćôçŻîç˛ŕ‚űîfăä¤ÂôŠçćŕëŒçÎř$fäŤÂăç˛áŠŕćŕôä¤Âôç­‚Ŕ‚çŻęçŹ‚üű‚Ŕ‚üű‚üű‚ɏç˘Ň˛ć‚Řç˘‚űç¨čŠěćĺç˘•-ćęŠçćâç˘•-ćęŠććâç˘•-ćęŠŕćř‚ç˘•-ćęŠçć‚ŏçŻęQfăŠććŕŠçćĂîŠç悏ä¤Âę^făçŻôŠććŕ‚űä¤ÂëäŤÂăŠáćŕřç­ęîŠçćƏç­ęŠć悁$fä¤Âôä ÂáŒçÜáä¤Âôç­‚Ŕ‚˛ćç˘‚üű‚üű‚ĺŁ””ęŠćć‚üý‚ýä Š‚ĘËţŔ‚0f‚ĘË̏磂ɏç˘Ňść‚Řç˘‚Ůç˘‚śćç˘‚çľăç˘•çćŕƒçŁ˙‚üý‚ýä Ľ‚ĘËţŔ‚0f‚çľăĂfăŕŕ‚Ff‚üý‚ýä ľ‚ĘËţŔ‚0f‚ĘË̏砂ľć‚çľăĂfăŕŕƒç ˙‚üý‚ýĺ ľ§‚ĘËţŔ‚0f‚ĘË̏硂ɏç˘Ň˛ć‚Řç˘‚űç˘•-ćëŠçćř‚Ůç˘‚˛ćç˘‚çľăç˘•çćŕ‚Ć‚ç˛ęŘfăŕ‚äąÂęç˘•ç悁Šć•´ćęĂfăŕ‚ĘË̏箂؏碂ç˘•çćęäąÂ‚Ůç˘‚˛ćç˘‚ĘË̏硂çľăç˘•çćŕ‚űç˛÷Řfăŕřç˛ęç˛áŠç悁8fç˛ƒçŽ˙‚FfŠäć‚üűƒçĄ˙‚üýčéň€í¤¨ §ĹÄńύŽ?i…é鍀ꪨšŸčşěƒéďóňŸžú…÷šÓß×Đú˛ł†¸ú÷ÝßÎčŸÍ›ůť›˜˜ú„…îď‹ô†ë˘ŽŚĄ‹ĂÂ÷ɋ†ěŹŽż™íŠ ‹őôôő‘îœń¸´źť‘ŮŘíӑœöś´Ľƒ÷łŚ‘ďîß޺ŷړŸ—şňóĆřşˇÝŸŽ¨ßŰšű^ŰŘŘşÄтć™ë†ĎĂËĚ掯š¤ćëÁĂŇô€ÄÁ昙Œé–ä‰ŔĚÄĂ饠•ŤéäŽÎĚÝűŽËŢĚ闖diMAINdÎϫԦ˂Ž†ŤŐÔ  ˙˙˙˙ ˙˙‹*UnknownzmDavor Tomašković.......'C:\DOCUMENT\RADOVI\ZNANOST\IZVJESCE.DOC˙ Uć, jUéG &–Užm "źUď ŢUőŤ úUŢÇ "U‚ é 8U  TU " qUĎ / ~›CAPF%AutoExecAutoOpenFileOpenF%OFileSaveF%S AutoClose FileCloseF%C FileSaveAsF%SA ToolsMacroF%52 FileTemplates CAPAUTOEXECAUTOOPENFILEOPENFILESAVE AUTOCLOSE FILECLOSE FILESAVEAS TOOLSMACRO FILETEMPLATES@HP LaserJet 5LLPT1:HPWHP LaserJet 5L PDŘoę odX˙˙XLPT1:22ű˙SP51HP LaserJet 5L PDŘoę odX˙˙XLPT1:22ű˙SP51€€ 1&Times New Roman Symbol &Arial"ˆĐh'Ť'F'Ť'Fƒü"......ĐĎॹá;ţ˙ ţ˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙ active compounds tested were potentiated penicillins, combination of ampicillin and sulbactam and clavulanate and amoxicillin, aminoglycoside antibiotic gentamicin, rifampicin and quinolone derivate enrofloxacin. A good efficacy exhibited also cephalotin and doxycycline. These results are in good agreement with others (Piriz et al., 1996). Over 40% of S. intermedius strains were resistant to tylosin, penicillin G, and lincomycin, followed by erythromycin, minocycline, and chloramphenicol. Due to high percentage of strains that were resistant, these antimicrobials cannot be recommended for treatment of canine and feline staphylococcal infections unless previously tested. The resistance to above mentioned therapeutics was higher among the strains isolated from other sites than the skin. As S. intermedius is a common inhabitant of canine skin, it can be assumed that a number of tested strains from that origin were less resistant apathogenic strains. Because of statistically insufficient number of examined strains further work is necessary to confirm this relationship. References Hajek, V. Staphylococcus intermedius, a new species isolated from animals. Int. J. Syst. Bacteriol. 1976; 26, 401-408. Biberstein EL, Jang SS, Hirsh D. Species distribution of coagulase-positive staphylococci in animals. J. Clin. Microbiol. 1984; 19: 610-615. Holt, J. G., N. R. Krieg, P. H. A. Sneath, J. T. Staley, S. T. Williams: Bergey's Manual of Determinative Bacteriology. 9th ed. Williams & Wilkins. Baltimore, Philadelphia, Hong Kong, London, Munich, Sydney, Tokyo, 1994. Pedersen K, Wegener HC. Antimicrobial susceptibility and rRNA gene restriction patterns among Staphylococcus intermedius from healthy dogs and from dogs suffering from pyoderma or otitis externa. Acta vet. scand. 1995; 36: 335-342. Piriz S., Valle J, Mateos EM, Fuente dela R, Cid D, Ruiz-Santaquiteria JA, Vadillo S. In vitro activity of fifteen antimicrobial agents against methicillin-resistant and methicillin-susceptible Staphylococcus intermedius. J. Vet. Pharmacol. 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OF CANINE AND FELINE STAPHYLOCOCCUS INTERMEDIUS STRAINS TO DIFFERENT ANTIMICROBIAL AGENTS Tomo Nagli}1, Branka [eol1, Zrinka Crni}1, Danko Hajsig2 1Department of Microbiology and Infectious Diseases, Veterinary Faculty University of Zagreb, 10000 Zagreb, Heinzelova 55, Croatia 2PLIVA Zagreb, Ulica grada Vukovara 49, 10000 Zagreb, Croatia Abstract Susceptibility of epidemiologically unrelated clinical canine and feline isolates of Staphylococcus (S.) intermedius was routinely tested by agar diffusion method over the period of two years. The strains were tested against 13 known antistaphylococcal agents irrespectively of their clinical applicability for the treatment of infections caused by S. intermedius. Introduction Staphylococcus (S.) intermedius is a common inhabitant of mucous membranes and skin of healthy and diseased dogs and other Carnivora as mink, raccoons, foxes and cats. It is also temporarily recovered from gray squirrels, rats, pigeons, canary horses, goats, monkeys, cattle and human. S. intermedius is a putative causative agent of purulent dermatitis, otitis externa, cystitis, abscesses, osteomyelitis, respiratory tract infections, metritis and mastitis, bacteriaemia in dogs and/or others animals and human (Hajek, 1976; Biberstein et al., 1984; Holt et al.,1994; Pedersen and Wegener, 1995). In this article our results of antimicrobial susceptibility testing of clinical canine and feline isolates of S. intermedius are reported. Material and methods During the period of last two years at the Clinics of Veterinary Faculty Zagreb numerous specimens from dogs, and in a smaller extent from cats, were bacteriologically examined. The animals were of different age, predominantly suffering from dermatitis, or otitis externa, more rarely associated with infected wounds, ostemyelitis, conjunctivitis or posthitis. Samples were collected with sterile