Naslov
Effect of oil industry "high density brines" estimated by Allium test

Autori
Vidaković-Cifrek, Željka ; Pavlica, Mirjana ; Tkalec, Mirta ; Regula, Ivan ; Papeš, Dražena

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
CROTOX 2000 - FINAL PROGRAMME and ABCTRACT BOOK / Prpić-Majić, Danica - Zagreb : Hrvatsko toksikološko društvo, 2000, 66-66

Skup
CROTOX 2000 - 2nd Croatian Congress of Toxicology (with international participation)

Mjesto i datum
Pula, Hrvatska, 09.04.2000. - 12.04.2000

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
calcium chloride; calcium bromide; Allium test

Sažetak
Saturated water solutions of calcium chloride, calcium bromide (densities 1.30 kg dm-3 and 1.61 kg dm-3, respectively) and their 1:1 mixture have been commonly used as oil industry "high density brines" for pressure control in oil wells. In some accidental situations, these solutions could end-up into environment and pollute nearby natural waters and agricultural areas. The cytotoxic effect of these solutions on the root tip cells of Allium ascalonicum auct. was evaluated using Allium test. The saturated water solutions of CaCl2, CaBr2 and their 1:1 mixture were added to tap water in amounts appropriate to achieve following concentrations: 0.025, 0.05, 0.075 and 0.1 mol dm-3. All tested solutions in concentrations 0.075 and 0.1 mol dm-3 caused significant inhibition of root growth. CaBr2 showed such effect already in concentration 0.05 mol dm-3. All three investigated solutions in all concentrations applied caused lower mitotic activity (mitotic index) in meristematic root tip cells in comparison with the control. Mitotic abnormalities were also noticed. The most of them were the consequence of spindle failure and chromosome stickiness. Furthermore, the cytoskeleton was investigated by indirect immunofluorescence to confirm that the most of abnormalities observed were the consequence of spindle failure. During that experiment, disturbed mitotic spindle and phragmoplast as well as irregular position of preprophase band, which are all protein structures, were found. In present study as well as in previous investigation of the same chemicals using Lemna test and Chlorella test, investigated samples showed certain effects on plants, so constant control of their presence in environment is needed.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

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