Naslov
Thin-layer Chromatographic Differentiation of Sphingolipid Bases and Complex Sphongolipids with Osmium Tetroxide

Autori
Kljajić, Ksenija ; Gojmerac, Astrid ; Proštenik, Mihovil

Izvornik
Acta Pharmaceutica Iugoslavica (0001-6667) 25 (1975), 1; 1-5

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
sphingolipid bases; complex sphingolipids; osmium tetroxide; thin-layer chromatography

Sažetak
A thin-layer chromatographic differentiation of the sphingolipid bases ceramides and cerebrosides, according to the number of the c=c double bonds, is described. The method is based on the substantial increase in polarity of the unsaturated lipid due to introduction, by oxidation with osmium tetroxide of two hydeoxy groups per double bond. The long-chain bases were N-acetylated before oxidation, whereas complex sphingolipids were oxidized without pretreatment. The difference in the Rf values (delta Rf) of unoxidized and the more polar oxidized material allows a clean-cut distinction of the long-chain bases and their complex derivatives (sphingolipids) with respecxt to the degree of unsaturation.

Izvorni jezik
Engleski

Znanstvena područja
Kemija

POVEZANOST RADA