Naslov
Antioxidant capacity evolution during maturation of cabbage varieties (Brassica oleracea and Brassica rapa)

Autori
Šamec, Dunja ; Piljac-Žegarac, Jasenka

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Book of Abstracts - YISAC 2009, Young Investigators Seminar on Analytical Chemistry / - Graz : Institute of Chemistry, Karl Franzens University, 2009, 55-56

Skup
Young Investigators Seminar on Analytical Chemistry 2009

Mjesto i datum
Graz, Austrija, 29.06.2009. - 02.07.2009

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
ACI; Chinese cabbage; flavonoids; phenols; white cabbage

Sažetak
Brassica oleracea cabbage cultivars are native to the Mediterranean region and southwestern Europe, however, B. oleracea forms are today grown for food everywhere in the world. Cultivar Varaždinski cabbage is especially popular in Croatia, with a detrimental role in the Croatian diet and agricultural system and widespread use in traditional medicine to treat acute inflammation and to promote rapid healing of peptic ulcers. The evolution of antioxidant activity as well as total phenol (TP, Folin-Ciocalteu method) and total flavonoid (TF, colorimetric assay with AlCl3) contents of white cabbage (Brassica olerace) cultivar Varaždinski (seed produced in the European Union - Italy, Croatia - Istra, and by a local producer in Varaždin), cultivar Ogulinski and Chinese cabbage (Brassica rapa) were analyzed each month during three months. The cabbage was grown under identical growing conditions in a greenhouse in north Croatia (Varaždin county) and every four weeks the leaves were collected, shredded and pure juice was subjected to analyses. All analyses were performed within 24 hours after juice extraction. Four weeks after planting, the TP and TF contents, as well as antioxidant capacity evaluated by all three methods did not show significantly different values between five analyzed cultivars. After 8 weeks the average TP content (3.95 g/L GAE) of cultivars was about 1.5 times higher in comparison to the average TP content after 4 weeks (2.60 g/L GAE). After 12 weeks, there was a two-fold increase in the average TP content compared to the levels detected after 4 weeks. The average TF content increased 2.1 and 2.7-fold after 8 and 12 weeks, respectively. The antioxidant potency composite index (ACI), giving equal weight to all three methods used to quantify antioxidant capacity. After 4 and 12 weeks the ACI value was the highest for cultivar Varaždinski (97.5 and 100) (local seed producer), while it was the highest for cultivar Ogulinski after 8 weeks (100). Positive correlation was found between the TP content and ACI values (r= 0.505, 0.741, 0.959) and between TF content and ACI (r= 0.620, 0.885, 0.980) after 4, 8, and 12 weeks indicating that polyphenolic content is highly correlated with the overall antioxidant capacity of studied B.oleracea and B. rapa cultivars. The monitoring will be continued until the cabbage cultivars reach full maturation (6 months).

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Biotehnologija

POVEZANOST RADA