Naslov
Differential host DNA methylation might be the cause of phytoplasma elimination upon the treatment with auxins

Autori
Ježić, Marin ; Leljak-Levanić, Dunja ; Ludwig-Müller, Jutta ; Mladinić, Marin ; Katić, Marija ; Ćurković-Perica, Mirna

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Abstrasđcrs of the 21st International Conference on Virus and other Graft Transmissible Diseases of Fruit Crops ; u: Berichte aus dem Julius Kühn-Institut / Julius Kühn-Institut - Braunschweig : Eigenverlag, 2009, 84-85

Skup
International Conference on Virus and other Graft Transmissible Diseases of Fruit Crops (21 ; 2009)

Mjesto i datum
Neustadt an der Weinstraße, Njemačka, 05.07.2009. - 10.07.2009

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
phytoplasma; elimination; methylation

Sažetak
Phytoplasmas from the class Mollicutes are wall-less, pleomorphic endocellular plant pathogenic bacteria that live in the phloem of their plant hosts. Phytoplasmas have reduced genomes of 530 – 1130 kb. Therefore, they are highly dependent on the intake of the nutrients form their hosts, cannot be grown on artificial media and cannot be inoculated mechanically on healthy plants to produce infection. It has been shown that a transfer of in vitro grown phytoplasma-infected Catharanthus roseus plantlets from medium supplemented with cytokinine, 6-benzylaminopurine (BA) to the one supplemented with auxins, indole-3-acetic acid (IAA) or indole-3-butyric acid (IBA) can induce remission of symptoms and even permanent elimination of ‘ Candidatus Phytoplasma asteris’ reference strain HYDB. To elucidate the possible mechanism of phytoplasma elimination from C. roseus shoots caused by IBA-treatment, we measured and compared endogenous auxins' levels and general methylation levels in healthy periwinkles, periwinkles infected with different ‘ Candidatus Phytoplasma’ species and phytoplasma-recovered periwinkles. Healthy shoots did not respond, or responded very weakly to exogenously added auxin, maintaining their phenotype, nominal levels of methylation and hormone concentrations. Phytoplasma-infection caused a change in endogenous levels of auxins in infected periwinkle shoots infected with different ‘ Candidatus Phytoplasma’ species, but general methylation levels were not statistically different from healthy plants except in the case of ‘ Ca. P. asteris’ , which was the phytoplasma strain eliminated from tissues when periwinkles were transferred to IBA containing medium. Therefore, low level of host genome methylation caused by ‘ Ca. P. asteris’ infection of periwinkles, statistically elevated after IBA-treatment, might be the cause of this phytoplasma elimination.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA