Naslov
Liquid chromatographic determination of some B-complex vitamins in different types of flours used in macrobiotic nutrition

Autori
Pajkin-Tučkar, Dubravka ; Mrkić, Vlatka, Jovanović, Karmela ; Vorkapić-Furač, Jasna

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Brašno-Kruh "97 / Ugarčić-Hardi, Žaneta - Osijek : Prehrambeno tehnološki fakultet Sveučilišta Josipa Jurja Strossmayera u Osijeku, 1997

Skup
I hrvatski kongres tehnologa proizvodnje i prerade brašna

Mjesto i datum
Opatija, Hrvatska, 13.11.1997. - 15.11.1997

Vrsta sudjelovanja
Poster

Vrsta recenzije
Nije recenziran

Ključne riječi
vitamini B-kompleksa; tekućinska kromatografija; brašno
(B-compelx vitamins; liquid chromatography; flour)

Sažetak
A sensitive and precise liquid chromatographic method has been used for the determination of total thiamin, riboflavin and niacin content in three four types used in macrobiotic nutrition. The arbitrariy selected samples were extracted with hydrochloric acid (thiamin and riboflavin) and calcium hydroxide (niacin) in autoclave, followed by an enzymatic hydrolysis with takadiastase (EC 3.2.1.1). Thiamin was oxidized to thiochrom, while riboflavin and niacin were chromatographed in the original form. After cleaning procedure on C18 cartridges (Superclean LC-C18) the samples were chromatographed on a reverse-phase C18 column by using different combinations of mobile phase (methanol, acetonitrile, acetate buffer, phosphate buffer, respectively) and two detectors (UV Diode Array and Flourichrom). All the data obtained by LC technique showed no significant differences in comparison with the values obtained by AOAC fluorometric method and agreed with the vitamins levels claimed by the manufactures, as well.

Izvorni jezik
Engleski

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