Naslov
Emergence of CTX-M 1 ESBL-producing Klebsiella pneumoniae strains in the Community

Autori
Vraneš, Jasmina ; Bedenić, Branka ; Bošnjak, Zrinka ; Marijan, Tatjana

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
International Journal of Antimicrobial Agents Vol. 34 Supplement 2 / Abstracts / - , 2009, S44-S45

Skup
26th International Congress of Chemotherapy and Infection

Mjesto i datum
Toronto, Kanada, 18.06.2009. - 21.06.2009

Vrsta sudjelovanja
Poster

Vrsta recenzije
Nije recenziran

Ključne riječi
Klebsiella pneumoniae; antibiotici; rezistencija; izvanbolničke infekcije
(Klebsiella pneumoniae; antibiotics; resistance; community-acquired infections)

Sažetak
Objective: Extended-spectrum beta-lactamase (ESBL)-producing Gram negative bacteria are an emergent cause of urinary tract infections in nonhospitalized patients in different countries. Studies on ESBLs in the outpatient populations reported CTX-M ESBLs to be the most prevalent. The aim of this study was the molecular characterization of ESBL-producing K. pneumoniae strains isolated from urine of outpatients in Zagreb region during the last five years. Methods: During the five-year study period a total of 2, 651 K. pneumoniae strains were isolated from urine of nonhospitalized patients with significant bacteriuria. ESBL production was detected by double-disk diffusion technique and by >3-dilution reduction in the minimal inhibitory concentration of ceftazidime in the presence of clavulanate. A total of 441 ESBL-producing K. pneumoniae strains (15.5%) were collected and 25 strains were characterised. Characterization of ESBLs was performed by PCR with specific primers for ESBLs and by sequencing of blaESBL genes. Results: A significant difference in frequencies of ESBL isolates was observed. In the first year of study only 4.9% of isolated strains were ESBL producers, while in the second year 17.3% ESBL strains were detected (p<0.01), and the frequency remain stabile within following years. Out of 25 characterized strains all strains yielded an amplicon with primers specific for SHV beta-lactamases. Five strains possessed an additional TEM beta-lactamase, and 11 strains possessed CTX-M beta-lactamases. Based on sequencing of blaCTX-M genes, enzymes of nine strains were identified as CTX-M 1 beta-lactamase. Conclusion: The study demonstrated community-associated emergence of CTX-M 1 beta-lactamase&#8211; producing K. pneumoniae strains. Further studies are necessary to investigate the possibility of clonal dissemination and hospital origin of those strains.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti, Kliničke medicinske znanosti, Javno zdravstvo i zdravstvena zaštita

POVEZANOST RADA