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Pregled bibliografske jedinice broj: 420422

Radioprotective effects of manganese-containing superoxide dismutase mimics on ataxia– telangiectasia cells

Pollard, Julianne M.; Reboucas, Julio S.; Durazof, Armando; Kos, Ivan; Fike, Francesca; Pannig, Moeen; Gralla, Edith Butler; Valentine, Joan Selverstone; Batinić-Haberle, Ines; Gatti, Richard A.
Radioprotective effects of manganese-containing superoxide dismutase mimics on ataxia– telangiectasia cells // Free Radical Biology and Medicine, 47 (2009), 3; 250-260 doi:10.1016/j.freeradbiomed.2009.04.018 (međunarodna recenzija, članak, znanstveni)

CROSBI ID: 420422 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Radioprotective effects of manganese-containing superoxide dismutase mimics on ataxia– telangiectasia cells

Autori
Pollard, Julianne M. ; Reboucas, Julio S. ; Durazof, Armando ; Kos, Ivan ; Fike, Francesca ; Pannig, Moeen ; Gralla, Edith Butler ; Valentine, Joan Selverstone ; Batinić-Haberle, Ines ; Gatti, Richard A.

Izvornik
Free Radical Biology and Medicine (0891-5849) 47 (2009), 3; 250-260

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
SOD mimics; Ataxia– telangiectasia; Radioprotection; Mn porphyrins; AEOL10112; AEOL10113; Mn salens; Mn cyclic polyamines; M40403; M40404; EUK-8; EUK-134; EUK-189; Free radicals

Sažetak
We tested several classes of antioxidant manganese compounds for radioprotective effects using human lymphoblastoid cells: six porphyrins, three salens, and two cyclic polyamines. Radioprotection was evaluated by seven assays: XTT, annexin V and propidium iodide flow cytometry analysis, γ -H2AX immunofluorescence, the neutral comet assay, dichlorofluorescein and dihydroethidium staining, resazurin, and colony survival assay. Two compounds were most effective in protecting wild-type and A-T cells against radiation-induced damage: MnMx-2-PyP-Calbio (a mixture of differently N-methylated MnT-2-PyP+ from Calbiochem) and MnTnHex-2-PyP. MnTnHex-2-PyP protected WT cells against radiation-induced apoptosis by 58% (p = 0.04), using XTT, and A-T cells by 39% (p = 0.01), using annexin V and propidium iodide staining. MnTnHex-2-PyP protected WT cells against DNA damage by 57% (p = 0.005), using γ -H2AX immunofluorescence, and by 30% (p < 0.01), using neutral comet assay. MnTnHex-2-PyP is more lipophilic than MnMx-2-PyP-Calbio and is also >10-fold more SOD-active ; consequently it is >50-fold more potent as a radioprotectant, as supported by six of the tests employed in this study. Thus, lipophilicity and antioxidant potency correlated with the magnitude of the beneficial radioprotectant effects observed. Our results identify a new class of porphyrinic radioprotectants for the general and radiosensitive populations and may also provide a new option for treating A-T patients.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Biologija, Farmacija



POVEZANOST RADA

Ustanove:
Farmaceutsko-biokemijski fakultet, Zagreb

Profili:

Avatar Url Ivan Kos (autor)

Avatar Url Ines Batinić-Haberle (autor)

Poveznice na cjeloviti tekst rada:

doi www.sciencedirect.com

Citiraj ovu publikaciju:

Pollard, Julianne M.; Reboucas, Julio S.; Durazof, Armando; Kos, Ivan; Fike, Francesca; Pannig, Moeen; Gralla, Edith Butler; Valentine, Joan Selverstone; Batinić-Haberle, Ines; Gatti, Richard A.
Radioprotective effects of manganese-containing superoxide dismutase mimics on ataxia– telangiectasia cells // Free Radical Biology and Medicine, 47 (2009), 3; 250-260 doi:10.1016/j.freeradbiomed.2009.04.018 (međunarodna recenzija, članak, znanstveni)
Pollard, J., Reboucas, J., Durazof, A., Kos, I., Fike, F., Pannig, M., Gralla, E., Valentine, J., Batinić-Haberle, I. & Gatti, R. (2009) Radioprotective effects of manganese-containing superoxide dismutase mimics on ataxia– telangiectasia cells. Free Radical Biology and Medicine, 47 (3), 250-260 doi:10.1016/j.freeradbiomed.2009.04.018.
@article{article, author = {Pollard, Julianne M. and Reboucas, Julio S. and Durazof, Armando and Kos, Ivan and Fike, Francesca and Pannig, Moeen and Gralla, Edith Butler and Valentine, Joan Selverstone and Batini\'{c}-Haberle, Ines and Gatti, Richard A.}, year = {2009}, pages = {250-260}, DOI = {10.1016/j.freeradbiomed.2009.04.018}, keywords = {SOD mimics, Ataxia– telangiectasia, Radioprotection, Mn porphyrins, AEOL10112, AEOL10113, Mn salens, Mn cyclic polyamines, M40403, M40404, EUK-8, EUK-134, EUK-189, Free radicals}, journal = {Free Radical Biology and Medicine}, doi = {10.1016/j.freeradbiomed.2009.04.018}, volume = {47}, number = {3}, issn = {0891-5849}, title = {Radioprotective effects of manganese-containing superoxide dismutase mimics on ataxia– telangiectasia cells}, keyword = {SOD mimics, Ataxia– telangiectasia, Radioprotection, Mn porphyrins, AEOL10112, AEOL10113, Mn salens, Mn cyclic polyamines, M40403, M40404, EUK-8, EUK-134, EUK-189, Free radicals} }
@article{article, author = {Pollard, Julianne M. and Reboucas, Julio S. and Durazof, Armando and Kos, Ivan and Fike, Francesca and Pannig, Moeen and Gralla, Edith Butler and Valentine, Joan Selverstone and Batini\'{c}-Haberle, Ines and Gatti, Richard A.}, year = {2009}, pages = {250-260}, DOI = {10.1016/j.freeradbiomed.2009.04.018}, keywords = {SOD mimics, Ataxia– telangiectasia, Radioprotection, Mn porphyrins, AEOL10112, AEOL10113, Mn salens, Mn cyclic polyamines, M40403, M40404, EUK-8, EUK-134, EUK-189, Free radicals}, journal = {Free Radical Biology and Medicine}, doi = {10.1016/j.freeradbiomed.2009.04.018}, volume = {47}, number = {3}, issn = {0891-5849}, title = {Radioprotective effects of manganese-containing superoxide dismutase mimics on ataxia– telangiectasia cells}, keyword = {SOD mimics, Ataxia– telangiectasia, Radioprotection, Mn porphyrins, AEOL10112, AEOL10113, Mn salens, Mn cyclic polyamines, M40403, M40404, EUK-8, EUK-134, EUK-189, Free radicals} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE

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