Naslov
Lack of risk of transmission of caprine arthritis-encephalitis virus (CAEV) after an appropriate embryo transfer procedure

Autori
Ali Al Ahmad, M.Z. ; Chebloune, Y. ; Bouzar, B.A. ; Baril, Gerard ; Bouvier, F. ; Chatagnon, G. ; Leboeuf, Bernard ; Pepin, M. ; Guibert, J.M. ; Russo, P. ; Manfredi, E. ; Martin, J. ; Fieni, F.

Izvornik
Theriogenology (0093-691X) 69 (2008), 4; 408-415

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
caprine arthritis-encephalitis virus; embryo transfer; IETS; polymerase chain reaction

Sažetak
The aim of this study was to demonstrate that embryo transfer can be used to produce CAEV-free kids from CAEV-infected biological mothers when appropriate procedure is implemented. Twenty-eight goats that had tested positive for CAEV using PCR on vaginal secretions were used as embryo donors. Embryos with intact-ZP were selected and washed 10 times ; they were then frozen and used for transfer into CAEV-free recipient goats. Nineteen of the 49 recipient goats gave birth, producing a total of 23 kids. Three blood samples were taken from each recipient goat, 10 days before, during, and 10 days after parturition ; these were tested for CAEVantibodies using ELISA and for CAEV proviral DNA using PCR. The mothers were then euthanized. Tissue samples were taken from the lungs, udder, and retromammary and prescapular lymph nodes. The kids were separated from their mothers at birth. Seven of them died. At 4 months of age, 16 kids were subjected to druginduced immunosuppression. Blood samples were taken every month from birth to 4 months of age ; samples were then taken on days 15, 21, and 28 after the start of the immunosuppressive treatment. The kids were then euthanized and tissue samples taken from the carpal synovial membrane, lung tissue, prescapular lymph nodes, inguinal and retromammary lymph nodes, and uterus. All samples from the 19 recipient goats and 23 kids were found to be negative for CAEVantibodies and/or CAEV proviral DNA. Under acute conditions for infection this study clearly demonstrates that embryo transfer can be safely used to produce CAEVfree neonates from infected CAEV donors.

Izvorni jezik
Engleski

Znanstvena područja
Veterinarska medicina

POVEZANOST RADA