Naslov
Antibody against Saccharomyces cerevisiae (ASCA) and antineutrophil cytoplasmic antibody (ANCA)in teh diagnosis of inflammatory bowel disease

Autori
Kocen, Dubravka ; Geets K., Mirjana ; Krznarić, Željko ; Vucelić, Boris ; Malenica, Branko

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
2008 Annual Meeting of the Croatian Immunological Society / Rabatić S. i sur. - Zagreb : Hrvatsko imunološko društvo, 2008

Skup
2008 Annual Meeting of the Croatian Immunological Society

Mjesto i datum
Šibenik, Hrvatska, 09.10.2008. - 12.10.2008

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
ANCA; ASCA; inflammatory bowel disease

Sažetak
"Classical" cytoplasmic antineutrophil cytoplasmic antibody (C-ANCA) and typical perinuclear antineutrophil cytoplasmic antibody (P-ANCA) have been known as sensitive immunoserologic markers of certain types of systemic vasculitis. A subset of ANCA, commonly referred to as "atypical" perinuclear antineutrophil cytoplasmic autoantibodies (a/P-ANCA)) are a well recognised marker for ulcerative colitis (UC). Antibody to the yeast Saccharomyces cerevisiae (ASCA) is a possible new marker associated with Crohn s disease (CD). The aim of the study was to examine the value of detecting ASCA and/or a/P-ANCA for the diagnosis of CD and UC. The presence of ANCA was detected on ethano-fixed cytospin preparations of peripheral blood neutrophils by indirect immunofluorescence (IIF). ASCA was performed using standardized indirect immunofluorescence on smears of Saccharomyces cerevisiae. ASCA antigenic specificity was analysed by ELISA using purified phosphopeptidomannans from the cell wall of Saccharomyces cerevisiae as an antigen. The a/P-ANCA were detected in 78% (21/27) of patients with UC, in 22% (8/35) of patients with CD and ASCA in 54 % (19/35) of patients with CD. All patients with UC were ASCA negative. The combination of a positive a/P-ANCA test and a negative ASCA test yielded a sensitivity, specificity and PPV of 78%, 77% and 72% respectively for UC. The combination of a positive ASCA test and a negative a/P-ANCA test yielded a sensitivity, specificity and PPV of 46%, 100% and 100% respectively for CD. All patients with other diarrheal illness were ASCA and a/P-ANCA negative. Antibodies against phosphopeptidomannans (PPM) in patients with CD were observed more freguently among those with active disease. Our results suggest that anti-PPM and a/P-ANCA antibodies are strongly associated with Crohn s disease and ulcerative colitis, respectively. Combination of both tests could aid the diagnosis of inflammatory bowel disease.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti

POVEZANOST RADA