Pregled bibliografske jedinice broj: 41785
Bile Salts Transformations and Cholesterol Assimilation by Enterococcus faecium L3
Bile Salts Transformations and Cholesterol Assimilation by Enterococcus faecium L3 // 9th European Congress on Biotechnology / Hofman Marcel, Anne Jozef (ur.).
Brisel: European Federation of Biotechnology, 1999. (poster, nije recenziran, sažetak, znanstveni)
CROSBI ID: 41785 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Bile Salts Transformations and Cholesterol Assimilation by Enterococcus faecium L3
Autori
Kos, Blaženka ; Šušković, Jagoda ; Matošić, Srećko
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni
Izvornik
9th European Congress on Biotechnology
/ Hofman Marcel, Anne Jozef - Brisel : European Federation of Biotechnology, 1999
Skup
9th European Congress on Biotechnology
Mjesto i datum
Bruxelles, Belgija, 11.07.1999. - 15.07.1999
Vrsta sudjelovanja
Poster
Vrsta recenzije
Nije recenziran
Ključne riječi
bile salts; cholesterol assimilation; E. faecium
Sažetak
Summary:Bile tolerance is an essential criterion in the selection of
#microbial strains for probiotic use. E. faecium L3 was grown in MRS broth
#supplemented with 0.3% oxgall and 0.2% of individual bile salts. It was
#shown bile salts hydrolase- and steroid 7a-dehidroxylase activities when
#was grown in the presence of individual glyco- and tauroconjugated bile
#salts. E. faecium L3 assimilated about 50% of the initial cholesterol #concentration when was grown in the presence of 0.3% of oxgall.
#*Materials and Methods:The bile tolerance was detected by measuring the
#absorbance (at 600 nm) during the growth of E. faecium in the presence of
#bile salts. Bile salts transformations were determined by thin-layer
#chromatography(1). Walker and Gilliland's technique was used for determination
#of free cholic acid liberated from the cultures during deconjugation(2). The
#concentration of free cholic acid in the supernatant was read from standard
#curve. Results were expressed as micromoles of cholic acid per ml of broth.
#The o-phthalaldehyde method for measuring cholesterol by Rudel and
#Morris(3)was used to determine the amount of cholesterol in spent broth.
#*Overview and discussion of results: If metabolic activity, multiplication, or
#colonization in the small intestine are required for optimal activity of the
#probiotic strains, then a bile tolerance is an essential criterion in the
#selection of microbial strains. The ability of E. faecium L3 to grow in the
#presence of bile salts exhibited good bile tolerance. When was grown in the
#presence of glyco- and tauroconjugated bile salts, E. faecium L3 was shown
#bile salts hydrolase- and steroid 7a-dehidroxylase activities. The
#cholesterol assimilation by in vitro experiments were frequently used to
#explain in vivo hypocholesterolemic effect. So, in the presence of oxgall,
#E. faecium L3 assimilated about 50% of the initial cholesterol concentration.
#The influence of individual conjugated and deconjugated bile salts on
#cholesterol assimilation were also studied. Better results were obtained with
#addition of conjugated than with deconjugated bile salts.
#*Conclusions:E. faecium L3 was shown tolerance to bile, deconjugation of
#sodium taurocholate and sodium glycodeoxycholate, and steroid
#7a-dehidroxylation of cholic acid. The ability of E. faecium L3 to lover
#cholesterol concentration was highest in MRS broth with 0.3% of oxgall.
#*References:
#(1)G.W.Tannock,M.P.Dashkevicz,S.D.Feighner,Appl.Environ.Microbiol.55(1989)1848
#(2)D.K. Walker,S.E. Gilliland, J. Dairy Sci. 76 (1993) 956-961.
#(3)L.L. Rudel, M.D. Morris, J. Lipid Res. 14 (1973) 364-366.
Izvorni jezik
Engleski
Znanstvena područja
Prehrambena tehnologija
