Naslov
SELDI-TOF as a method for biomarker discovery in the urine of aristolochic-acid-treated mice

Autori
Huang, Feilei ; Clifton, James ; Yang, Xinli ; Rosenquist, Thomas ; Hixson, Douglas ; Kovač, Spomenka ; Josić, Djuro

Izvornik
Electrophoresis (0173-0835) 30 (2009), S.I.; 1168-1174

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Aristolochic acid; Chinese herbs nephropathy; endemic nephropathy; SELDITOF
(Chinese herbs nephropathy; endemic nephropathy; SELDITOF)

Sažetak
Aristolochic acids (AAs) present in Aristolochia plants are substances responsible for Chinese herbs nephropathy. Recently, strong indications have also been presented, which dietary poisoning with AA is responsible for endemic (Balkan) nephropathy, an enigmatic renal disease that affects rural population living in some countries in Southeastern Europe. A mouse model was applied to follow the effects of two forms of AA, AAI and AAII. SDS-PAGE and SELDI-TOF mass spectrometry with normal phase chips were used to evaluate changes in the urine of treated animals. These two methods are demonstrated to be comparable. The use of SELDI-TOF MS for rapid analysis of a large number of samples and the combination of this method with nano-LC-ESI MS/MS for protein identification were demonstrated. Biomarker discovery after analysis of large cohort of endemic nephropathy patients will be the final aim of these investigations

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Biologija, Biotehnologija

POVEZANOST RADA