Pregled bibliografske jedinice broj: 4130
Kinetika inhibicije acetilkolinesteraze diklorvosom i terbufosom u mozgu štakora
Kinetika inhibicije acetilkolinesteraze diklorvosom i terbufosom u mozgu štakora // CROTOX"96 - 1. Hrvatski toksikološki kongres s međunarodnim sudjelovanjem / Kniewald, Jasna ; Prpić-Majić, Danica (ur.).
Zagreb: Hrvatsko toksikološko društvo, 1996. (poster, domaća recenzija, sažetak, znanstveni)
CROSBI ID: 4130 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Kinetika inhibicije acetilkolinesteraze diklorvosom i terbufosom u mozgu štakora
(Kinetics of acethylcholinesterase inhibition by dichlorvos and terbufos in rat brain)
Autori
Jakominić, Mihela ; Matjeta, Anita ; Šimić, Branimir ; Kniewald, Jasna
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni
Izvornik
CROTOX"96 - 1. Hrvatski toksikološki kongres s međunarodnim sudjelovanjem
/ Kniewald, Jasna ; Prpić-Majić, Danica - Zagreb : Hrvatsko toksikološko društvo, 1996
Skup
CROTOX"96 - 1. Hrvatski toksikološki kongres
Mjesto i datum
Zagreb, Hrvatska, 17.04.1996. - 19.04.1996
Vrsta sudjelovanja
Poster
Vrsta recenzije
Domaća recenzija
Ključne riječi
acetilkolinesteraza; diklorvos; terbufos; mozak štakora
(acethylcholinesterase; dichlorvos; terbufos; rat brain)
Sažetak
The exposure to organophosphorus pesticides lead to cholinesterase inhibition and cause an accumulation of acetylcholine in the central nervous system. The effect of dichlorvos (2,2-dichlorovinyl dimethyl phosphate) and terbufos (S-tert-butylthiomethyl-O,O-diethyl phosphorodithioate) on the activity of acetylcholinesterase (AChE) in male rat brain cytosol was studied. The K_M and v_max values for AChE were determined (K_M 4,21-7,46 x 10^-5 M; v_max 0,0221-0,0356 x 10^-6 mol min^-1 mg^-1). Activity of AChE was measured by the method of Ellman et al.^1.
In vitro the influence of the various concentrations of dichlorvos (0,0025-0,1000 mM) and terbufos (0,01-0,25 mM) were studied. For dichlorvos v_max value was 0,0259ą0,0003 x 10^-6 mol min^-1 mg^-1 and K_M 0,354-0,416 x 10^-3 M, and for terbufos vmax 0,0208ą0,0014 x 10^-6 mol min^-1 mg^-1 and K_M 0,0457-0,1757 x 10^-3 M. A kinetic study of in vitro inhibition of AChE in the rat brain by dichlorvos and terbufos showed its competitive nature. K_i values were for dichlorvos 0,0059 x 10^-3 M and for terbufos 0,114 x 10^-3 M. Hill"s coefficients (n_H) were for dichlorvos 1,01 and for terbufos 0,92, that suggested one molecule of compound reacted with one molecule of AChE. Values of I_50 were for dichlorvos 3,14 microM and for terbufos 220,33 microM.
References: 1. Ellman, G.L., Courtney, K.D., Andres, V., Featherstone, R.M., Biochem. Pharmacol. 7 (1961) 88.
Izvorni jezik
Engleski
Znanstvena područja
Prehrambena tehnologija
