Naslov
Primary lung cancer and TNF-alpha gene polymorphisms: A case-control study in a Croatian population
(Primary lung cancer and TNF-alfa gene polymorphisms:A case-control study in a Croatian population)

Autori
Flego, Veljko ; Radojčić Badovinac, Anđelka ; Bulat-Kardum, Ljiljana ; Matanić, Dubravka ; Crnić-Martinović, Marija ; Kapović, Miljenko ; Ristić, Smiljana

Izvornik
Medical science monitor (1234-1010) 15 (2009), 7; 361-365

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Lung cancer ; TNF-alfa polymorphism ; Tumor type ; Tumor stage ; Caucasian population ; Cancer susceptibility

Sažetak
Tumor necrosis factor-a (TNF-a) is a multifunctional cytokine involved in the pathogenesis of various infl ammatory and malignant diseases. Previous studies investigating the role of the TNF-a gene polymorphisms in lung cancer have generated contradictory results. The present study investigated whether the TNF-a-308 and TNF-a-238 polymorphisms are associated with risk and/or severity of disease in Croatian lung cancer patients. This is the fi rst study in a Caucasian population to analyze the infl uence of these two polymorphisms on multiple types of lung cancer. Material/Methods: In a case-control study, lung cancer patients (n=230) and appropriate age- and sex-matched controls (n=230) were genotyped by the polymerase chain reaction/restriction fragment length polymorphism method. Allele and genotype frequencies were estimated by gene counting. The chisquared test was used to compare the observed numbers of different TNF-a genotypes for the population with those predicted by Hardy-Weinberg equilibrium. Differences in genotype and allele distributions in the patient and control groups were analyzed for statistical signifi cance using the chi-squared test or Fisher’ s exact test as appropriate. Results: There were no signifi cant differences in the genotype and allele frequencies for the TNF-a-308 and TNF-a-238 polymorphisms between lung cancer patients and controls. Furthermore, no association between the genotypes and different stages of lung cancer was detected. Conclusions: This study indicates that the TNF-a-308 and TNF-a-238 polymorphisms do not infl uence susceptibility to or severity of lung cancer in a Croatian population.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti, Kliničke medicinske znanosti

POVEZANOST RADA