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Pregled bibliografske jedinice broj: 412040

Alternative pig liver esterase (APLE)- cloning, identification and functional expression in Pichia pastoris of a versatile new biocatalyst

Hermann, Manuela; Kietzmann, Martin U.; Ivančić, Mirela; Zenzmaier, Christoph; Luiten, Ruud G.; Skranc, Wolfgang; Wubbolts, Marcel; Winkler, Margit; Birner-Gruenberger, Ruth; Pichler, Harald; Schwab, Helmut
Alternative pig liver esterase (APLE)- cloning, identification and functional expression in Pichia pastoris of a versatile new biocatalyst // Journal of biotechnology, 133 (2008), 3; 301-310 doi:10.1016/j.jbiotec.2007.10.010 (međunarodna recenzija, članak, znanstveni)

CROSBI ID: 412040 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Alternative pig liver esterase (APLE)- cloning, identification and functional expression in Pichia pastoris of a versatile new biocatalyst
(Alternative pig liver esterase (APLE) - cloning, identification and functional expression in Pichia pastoris of a versatile new biocatalyst)

Autori
Hermann, Manuela ; Kietzmann, Martin U. ; Ivančić, Mirela ; Zenzmaier, Christoph ; Luiten, Ruud G. ; Skranc, Wolfgang ; Wubbolts, Marcel ; Winkler, Margit ; Birner-Gruenberger, Ruth ; Pichler, Harald ; Schwab, Helmut

Izvornik
Journal of biotechnology (0168-1656) 133 (2008), 3; 301-310

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
biocatalysis; pig liver esterase; kinetic resolution; heterologous expression; Pichia pastoris; periplasm

Sažetak
Isolated from pig liver as a crude, inhomogeneous enzyme fraction, pig liver esterase (PLE) was found to metabolize a wide range of substrates ; often in a highly stereoselective manner. This crude esterase preparation, however, contains several iso-enzymes at proportions varying from batch to batch. Racemic methyl-(4E)-5-chloro-2-isopropyl-4-pentenoate is cleaved enantioselectively by crude PLE, but not by recombinantly expressed gamma-isoform of PLE. Concluding that another PLE iso-enzyme must carry the relevant activity, we cloned and sequenced cDNAs of several PLE isoforms and functionally expressed them in Pichia pastoris. One novel isoform termed alternative pig liver esterase (APLE) was found to hydrolyze methyl-(2R, 4E)-5-chloro-2-isopropyl-4-pentenoate in a highly stereoselective manner (E > 200). When heterologously expressed and directed for secretion in P. pastoris, APLE was found to be localized in the periplasm. The presence or absence of a putative C-terminal ER retention signal did neither influence functional expression nor cellular localization. The recombinant enzyme, purified by ion exchange chromatography, had a specific activity of 36 U per mg protein towards racemic methyl-(4E)-5-chloro-2-isopropyl-4-pentenoate.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija



POVEZANOST RADA

Projekti:
058-0581990-2004 - Hibridni integrirani bioprocesi i održivost proizvodnje organskih otapala (Šantek, Božidar, MZOS ) ( CroRIS)

Ustanove:
Prehrambeno-biotehnološki fakultet, Zagreb

Profili:

Avatar Url Mirela Ivančić Šantek (autor)

Poveznice na cjeloviti tekst rada:

doi

Citiraj ovu publikaciju:

Hermann, Manuela; Kietzmann, Martin U.; Ivančić, Mirela; Zenzmaier, Christoph; Luiten, Ruud G.; Skranc, Wolfgang; Wubbolts, Marcel; Winkler, Margit; Birner-Gruenberger, Ruth; Pichler, Harald; Schwab, Helmut
Alternative pig liver esterase (APLE)- cloning, identification and functional expression in Pichia pastoris of a versatile new biocatalyst // Journal of biotechnology, 133 (2008), 3; 301-310 doi:10.1016/j.jbiotec.2007.10.010 (međunarodna recenzija, članak, znanstveni)
Hermann, M., Kietzmann, M., Ivančić, M., Zenzmaier, C., Luiten, R., Skranc, W., Wubbolts, M., Winkler, M., Birner-Gruenberger, R., Pichler, H. & Schwab, H. (2008) Alternative pig liver esterase (APLE)- cloning, identification and functional expression in Pichia pastoris of a versatile new biocatalyst. Journal of biotechnology, 133 (3), 301-310 doi:10.1016/j.jbiotec.2007.10.010.
@article{article, author = {Hermann, Manuela and Kietzmann, Martin U. and Ivan\v{c}i\'{c}, Mirela and Zenzmaier, Christoph and Luiten, Ruud G. and Skranc, Wolfgang and Wubbolts, Marcel and Winkler, Margit and Birner-Gruenberger, Ruth and Pichler, Harald and Schwab, Helmut}, year = {2008}, pages = {301-310}, DOI = {10.1016/j.jbiotec.2007.10.010}, keywords = {biocatalysis, pig liver esterase, kinetic resolution, heterologous expression, Pichia pastoris, periplasm}, journal = {Journal of biotechnology}, doi = {10.1016/j.jbiotec.2007.10.010}, volume = {133}, number = {3}, issn = {0168-1656}, title = {Alternative pig liver esterase (APLE)- cloning, identification and functional expression in Pichia pastoris of a versatile new biocatalyst}, keyword = {biocatalysis, pig liver esterase, kinetic resolution, heterologous expression, Pichia pastoris, periplasm} }
@article{article, author = {Hermann, Manuela and Kietzmann, Martin U. and Ivan\v{c}i\'{c}, Mirela and Zenzmaier, Christoph and Luiten, Ruud G. and Skranc, Wolfgang and Wubbolts, Marcel and Winkler, Margit and Birner-Gruenberger, Ruth and Pichler, Harald and Schwab, Helmut}, year = {2008}, pages = {301-310}, DOI = {10.1016/j.jbiotec.2007.10.010}, keywords = {biocatalysis, pig liver esterase, kinetic resolution, heterologous expression, Pichia pastoris, periplasm}, journal = {Journal of biotechnology}, doi = {10.1016/j.jbiotec.2007.10.010}, volume = {133}, number = {3}, issn = {0168-1656}, title = {Alternative pig liver esterase (APLE) - cloning, identification and functional expression in Pichia pastoris of a versatile new biocatalyst}, keyword = {biocatalysis, pig liver esterase, kinetic resolution, heterologous expression, Pichia pastoris, periplasm} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus

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